Testing Data
Mouse GAPDH Monoclonal Antibody | anti-GAPDH antibody
Mouse anti GAPDH antibody
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI Description
catalyzes the interconversion of D-glyceraldehyde 3-phosphate, phosphate, and NAD(+) to 3-phospho-D-glyceroyl phosphate and NADH; may contribute to apoptosis [RGD]
Uniprot Description
Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Ref.3 Ref.13 Ref.15
Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure: Homotetramer. Interacts with EIF1AD, USP25, PRKCI and WARS. Interacts with TPPP; the interaction is direct
By similarity. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Ref.13 Ref.14 Ref.15
Subcellular location: Cytoplasm › cytosol. Nucleus. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Ref.13 Ref.14 Ref.15
Tissue specificity: High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. Ref.9
Post-translational modification: S-nitrosylation of Cys-150 leads to interaction with SIAH1, followed by translocation to the nucleus. The effect of S-nitrosylation on enzymatic activity is unclear: according to some authors, it inhibits enzymatic activity and increases endogenous ADP-ribosylation, inhibiting the enzyme in a non-reversible manner (Ref.12). According to others, it does not affect glycolysis (Ref.13). ADP-ribosylation is likely to be a pathophysiological event associated with inhibition of gluconeogenesis.ISGylated
Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Research Articles on GAPDH
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Product Notes
The GAPDH gapdh (Catalog #AAA462002) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti GAPDH antibody reacts with Human, Rat, Mouse, Dog, Fish and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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