Mouse GAP43 Monoclonal Antibody | anti-GAP43 antibody

GAP43 (N-terminal region) Antibody

Cat. Num. AAA474372

• Gene Names: GAP43; B-50; PP46
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA, Immunocytochemistry, Immunohistochemistry
• Synonyms: GAP43; Monoclonal Antibody; GAP43 (N-terminal region) Antibody; GAP43 (N-terminal region); anti-GAP43 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M333
• Specificity: This antibody detects a 43kDa* protein corresponding to the molecular mass of GAP43 on SDS-PAGE immunoblots of adult mouse brain.
• Form/Format: Mouse monoclonal, protein A purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-GAP43 antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:2000; ICC: 1:50; IHC: 1:400; WB: 1:1000
• Immunogen: Clone (M333) was generated from a sequence corresponding to amino acids in the N-terminal region of the human GAP43. This sequence has high homology to rat and mouse GAP43.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of mouse brain lysate. The blot was probed with mouse monoclonal anti-GAP43 (N-terminal region) antibody at 1:500 (lane 1) or 1:1000 (lane 2).)

Immunohistochemistry (IHC)

(Formalin fixed, citric acid treated parafin sections of E7 chick spinal cord. Sections were probed with anti-GAP43 (GM3331) then anti-mouse:HRP before detection using DAB. (Image provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).)

Related Product Information for anti-GAP43 antibody

GAP43 (B-50, F1, neuromodulin) is a phosphoprotein found in the presynaptic membranes of neurons, where it may have important roles in the maintenance of long-term potentiation and synaptic plasticity. Regulation of GAP43 activity may occur through PKC phosphorylation of Ser-41, which occurs during neurotransmitter release in mature neurons. Calmodulin may be a negative regulator of GAP43, since it binds to GAP43 when Ca2+ is low and neuronal activity is minimal. GAP43 is a major component of the growth cone in axons of developing neurons. Thus, it may have roles in axon development and synaptogenesis.

References

Gianotti, C. et al. (1992) Neuron 8:843.
McIntosh, H. et al. (1989) Visual Neuroscience 2:583.
Skene, J.H., et al. (1986) Science 233:783.

NCBI and Uniprot Product Information

• NCBI GI #: 194248057
• NCBI GeneID: 2596
• NCBI Accession #: NP_001123536.1
• NCBI GenBank Nucleotide #: NM_001130064.1
• UniProt Accession #: P17677; A8K0Y4
• Molecular Weight: 24,803 Da
• NCBI Official Full Name: neuromodulin isoform 1
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: GAP43
• NCBI Official Synonym Symbols: B-50; PP46
• NCBI Protein Information: neuromodulin; protein F1; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43
• UniProt Protein Name: Neuromodulin
• Protein Family: Neuromodulin
• UniProt Gene Name: GAP43
• UniProt Entry Name: NEUM_HUMAN

NCBI Description

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: filopodium membrane; axon; cytoplasm; postsynaptic density; plasma membrane; cell junction

Molecular Function: calmodulin binding; protein binding

Biological Process: cell fate commitment; glial cell differentiation; tissue regeneration; regulation of growth; axon choice point recognition; protein kinase C activation; response to wounding; regulation of filopodium formation