Mouse GAP43 Monoclonal Antibody | anti-GAP43 antibody

GAP43 (Neuromodulin, Axonal Membrane Protein GAP-43, Growth-associated Protein 43, Neural Phosphoprotein B-50, pp46)

Cat. Num. AAA633050

• Gene Names: GAP43; B-50; PP46
• Reactivity: Chicken, Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: GAP43; Monoclonal Antibody; GAP43 (Neuromodulin; Axonal Membrane Protein GAP-43; Growth-associated Protein 43; Neural Phosphoprotein B-50; pp46); Anti -GAP43 (Neuromodulin; anti-GAP43 antibody

• Host: Mouse
• Reactivity: Chicken, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 10K231
• Specificity: Recognizes rat GAP43. Species Crossreactivity: human, mouse, chicken, snake.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute in sterile ddH2O, PBS.

• Applicable Applications for anti-GAP43 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-1ug/ml; Immunohistochemistry (paraffin, frozen): 1-2ug/ml
• Immunogen: GAP-43 from neonatal rat forebrain membranes.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with 200ul sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-GAP43 antibody

GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells.It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.

Product Categories/Family for anti-GAP43 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 119600000
• NCBI GeneID: 2596
• UniProt Accession #: P17677; Q5U058; Q6LB24
• Molecular Weight: 24,803 Da
• NCBI Official Full Name: growth associated protein 43
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: GAP43
• NCBI Official Synonym Symbols: B-50; PP46
• NCBI Protein Information: neuromodulin; protein F1; OTTHUMP00000165225; OTTHUMP00000165226; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43
• UniProt Protein Name: Neuromodulin
• Protein Family: Neuromodulin
• UniProt Gene Name: GAP43
• UniProt Entry Name: NEUM_HUMAN

NCBI Description

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: filopodium membrane; axon; postsynaptic density; cytoplasm; plasma membrane; cell junction

Molecular Function: calmodulin binding; protein binding

Biological Process: glial cell differentiation; cell fate commitment; tissue regeneration; regulation of growth; axon choice point recognition; protein kinase C activation; response to wounding; regulation of filopodium formation