Goat TR3 ORPHAN RECEPTOR Polyclonal Antibody | anti-NR4A1 antibody

GOAT ANTI HUMAN TR3 ORPHAN RECEPTOR

Cat. Num. AAA224277

• Gene Names: NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• Reactivity: Dog (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: ELISA, Western Blot
• Synonyms: TR3 ORPHAN RECEPTOR; Polyclonal Antibody; GOAT ANTI HUMAN TR3 ORPHAN RECEPTOR; anti-NR4A1 antibody

• Host: Goat
• Reactivity: Dog (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises human TR3 orphan receptor, otherwise known as NUR77, an immediate-early orphan nuclear receptor, and member of the nuclear receptor hormone family, encoded by the NR4A1 gene. ; TR3 orphan receptor plays a role in the mediation of macrophage inflammatory responses, and in apoptosis and cell proliferation, through the binding to a variety of response elements. TR3 orphan receptor has been shown to bind to the anti-apoptotic protein Bcl-2, and can be induced by both physiological and physical stress-related stimuli, including tumour necrosis factor (TNF), inflammatory cytokines and magnetic fields. ; This item is expected to recognise isoform a of TR3 orphan receptor.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 325

• Applicable Applications for anti-NR4A1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 60kDa in human thyroid gland cell lysates.; ELISA: Minimum Dilution: 1/32000; Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 3.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Synthetic peptide sequence C-GRLPSKPKQPPDAS from the internal region of TR3 orphan receptor (NP_002126.2; NP_775180.1).; Buffer Solution
• Antiserum Preparation: Antiserum to human TR3 orphan receptor was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Human thyroid cell lysate probed with Goat anti Human TR3 orphan receptor (MBS224277))

Related Product Information for anti-NR4A1 antibody

MBS224277 specifically recognises human TR3 orphan receptor, otherwise known as NUR77, an immediate-early orphan nuclear receptor, and member of the nuclear receptor hormone family, encoded by the NR4A1 gene. TR3 orphan receptor plays a role in the mediation of macrophage inflammatory responses, and in apoptosis and cell proliferation, through the binding to a variety of response elements. TR3 orphan receptor has been shown to bind to the anti-apoptotic protein Bcl-2, and can be induced by both physiological and physical stress-related stimuli, including tumour necrosis factor (TNF), inflammatory cytokines and magnetic fields. MBS224277 is expected to recognise isoform a of TR3 orphan receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 320202956
• NCBI GeneID: 3164
• NCBI Accession #: NP_001189162.1
• NCBI GenBank Nucleotide #: NM_001202233.1
• UniProt Accession #: P22736; Q15627; Q53Y00; Q6IBU8; B4DML7
• Molecular Weight: 33,754 Da
• NCBI Official Full Name: nuclear receptor subfamily 4 group A member 1 isoform 2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 4, group A, member 1
• NCBI Official Symbol: NR4A1
• NCBI Official Synonym Symbols: HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• NCBI Protein Information: nuclear receptor subfamily 4 group A member 1; ST-59; TR3 orphan receptor; early response protein NAK1; growth factor-inducible nuclear protein N10; hormone receptor; nerve growth factor IB nuclear receptor variant 1; nuclear hormone receptor NUR/77; orphan nuclear receptor HMR; orphan nuclear receptor TR3; steroid receptor TR3; testicular receptor 3
• UniProt Protein Name: Nuclear receptor subfamily 4 group A member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR4A1
• UniProt Synonym Gene Names: GFRP1; HMR; NAK1; Nur77
• UniProt Entry Name: NR4A1_HUMAN

NCBI Description

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein.

Protein type: DNA-binding; Nuclear receptor; Apoptosis

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; signal transduction; cell migration during sprouting angiogenesis; innate immune response; positive regulation of endothelial cell proliferation; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling