Immunohistochemistry (IHC)
(Fig.1. IHC-P staining of Human thyroid tissue paraffin-embedded, diluted at 1:200.)
Mouse anti-Human Galectin-3 Monoclonal Antibody | anti-Gal-3 antibody
Galectin-3 Monoclonal Antibody
IF: 1:100-1:200
IHC-P: 1:200
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Immunohistochemistry (IHC)
(Fig.1. IHC-P staining of Human thyroid tissue paraffin-embedded, diluted at 1:200.)
Immunohistochemistry (IHC)
(Fig.1. IHC-P staining of Human thyroid tissue paraffin-embedded, diluted at 1:200.)
Western Blot (WB)
(Fig.2. Western blot analysis of Hela, diluted at 1:3000.)
Western Blot (WB)
(Fig.2. Western blot analysis of Hela, diluted at 1:3000.)
Immunofluorescence (IF)
(Fig.3. Immunofluorescence analysis of human lung cancer tissue. 1, Galectin-3 Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Immunofluorescence (IF)
(Fig.3. Immunofluorescence analysis of human lung cancer tissue. 1, Galectin-3 Monoclonal Antibody (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
Uniprot Description
Galectin-3: galactose-specific lectin which binds IgE. Expressed at a high level in the colonic epithelium. Also abundant in activated macrophages.
Protein type: Cell surface; Motility/polarity/chemotaxis; Extracellular matrix
Chromosomal Location of Human Ortholog: 14q22.3
Cellular Component: extracellular matrix; spliceosome; extracellular space; proteinaceous extracellular matrix; membrane; mitochondrial inner membrane; cytoplasm; plasma membrane; immunological synapse; nucleus; external side of plasma membrane
Molecular Function: protein binding; laminin binding; IgE binding; carbohydrate binding; chemoattractant activity
Biological Process: monocyte chemotaxis; neutrophil chemotaxis; extracellular matrix organization and biogenesis; epithelial cell differentiation; positive chemotaxis; RNA splicing; negative regulation of endocytosis; regulation of T cell proliferation; eosinophil chemotaxis; negative regulation of T cell receptor signaling pathway; innate immune response; mRNA processing; skeletal development; macrophage chemotaxis
Research Articles on Gal-3
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Product Notes
The Gal-3 lgals3 (Catalog #AAA9700268) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Galectin-3 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Galectin-3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. WB: 1:2000 IF: 1:100-1:200 IHC-P: 1:200. Researchers should empirically determine the suitability of the Gal-3 lgals3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galectin-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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