Western Blot (WB)
(Western blot analysis of lysates from Hela,U-251 MG cell line (from left to right), using FUBP3 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
Mouse anti-Human FUBP3 Monoclonal Antibody | anti-FUBP3 antibody
FUBP3 Antibody (N-term)
Gene Names
FUBP3; FBP3
Reactivity
Human
Applications
Western Blot, Immunofluorescence, ELISA
Synonyms
FUBP3; Monoclonal Antibody; FUBP3 Antibody (N-term); FBP3; Far upstream element-binding protein 3; anti-FUBP3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
Purified His-tagged FUBP3 protein was used to produced this monoclonal antibody.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
572
Applicable Applications for anti-FUBP3 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA( EIA)
Application Notes
WB: 1:1000
IF: 1:25
IF: 1:25
Antigen Source
Human
Antigen Region
1-241
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysates from Hela,U-251 MG cell line (from left to right), using FUBP3 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from Hela,U-251 MG cell line (from left to right), using FUBP3 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with FUBP3 Antibody (N-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with FUBP3 Antibody (N-term) . It was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-FUBP3 antibody
May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
References
Davis-Smyth T., et al. J. Biol. Chem. 271:31679-31687(1996). Humphray S.J., et al. Nature 429:369-374(2004). Bienvenut W.V., et al. Submitted (DEC-2008) to UniProtKB. Lubec G., et al. Submitted (DEC-2008) to UniProtKB. Matsuoka S., et al. Science 316:1160-1166(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
61640
NCBI Official Full Name
far upstream element-binding protein 3
NCBI Official Synonym Full Names
far upstream element binding protein 3
NCBI Official Symbol
FUBP3
NCBI Official Synonym Symbols
FBP3
NCBI Protein Information
far upstream element-binding protein 3
UniProt Protein Name
Far upstream element-binding protein 3
Protein Family
UniProt Gene Name
FUBP3
UniProt Synonym Gene Names
FBP3; FUSE-binding protein 3
UniProt Entry Name
FUBP3_HUMAN
Research Articles on FUBP3
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Product Notes
The FUBP3 fubp3 (Catalog #AAA9231769) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FUBP3 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FUBP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA( EIA). WB: 1:1000 IF: 1:25. Researchers should empirically determine the suitability of the FUBP3 fubp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FUBP3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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