Mouse FTO Monoclonal Antibody | anti-FTO antibody

Mouse Monoclonal [clone 5-2H10] (IgG) to Rat FTO

Cat. Num. AAA246395

• Gene Names: FTO; ALKBH9
• Reactivity: Bovine, Dog, Horse, Human, Mouse, Primate, Rat, Sheep
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Protein G Purified
• Synonyms: FTO; Monoclonal Antibody; Mouse Monoclonal [clone 5-2H10] (IgG) to Rat FTO; Anti-FTO Antibody (clone 5-2H10) IHC-plus; KIAA1752; Protein fto; Rat FTO; anti-FTO antibody

• Host: Mouse
• Reactivity: Bovine, Dog, Horse, Human, Mouse, Primate, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 5-2H10
• Specificity: Specific for the ~58 kDa Fto protein in Western blots of rat testes lysate. The antibody has also been used for immunocytochemistry with neuronal progenitor cells.
• Purity/Purification: Protein G Purified
• Form/Format: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol.
• Sequence Length: 505

• Applicable Applications for anti-FTO antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF (1:100), IHC-P (1:50), WB (1:1000)
• Target Species: Rat
• Immunogen Description: Synthetic peptide from the N-terminal region of rat Fto
• Immunogen Type: Synthetic peptide
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-FTO antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Western Blot (WB)

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NCBI and Uniprot Product Information

• NCBI GI #: 122937263
• NCBI GeneID: 79068
• NCBI Accession #: NP_001073901.1
• NCBI GenBank Nucleotide #: NM_001080432.2
• UniProt Accession #: Q9C0B1; Q0P676; Q7Z785; A2RUH1; B2RNS0
• Molecular Weight: 12,218 Da
• NCBI Official Full Name: alpha-ketoglutarate-dependent dioxygenase FTO
• NCBI Official Synonym Full Names: fat mass and obesity associated
• NCBI Official Symbol: FTO
• NCBI Official Synonym Symbols: ALKBH9
• NCBI Protein Information: alpha-ketoglutarate-dependent dioxygenase FTO; protein fto; AlkB homolog 9; fat mass and obesity-associated protein
• UniProt Protein Name: Alpha-ketoglutarate-dependent dioxygenase FTO
• Protein Family: Alpha-ketoglutarate-dependent dioxygenase
• UniProt Gene Name: FTO
• UniProt Synonym Gene Names: KIAA1752
• UniProt Entry Name: FTO_HUMAN

NCBI Description

This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]

Uniprot Description

FTO: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD). A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years. Belongs to the fto family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: nuclear speck; nucleus

Molecular Function: ferrous iron binding; DNA-N1-methyladenine dioxygenase activity

Biological Process: DNA dealkylation; RNA repair; regulation of multicellular organism growth; thermoregulation

Disease: Growth Retardation, Developmental Delay, Coarse Facies, And Early Death