Mouse anti-Human, Mouse FoxP3 Monoclonal Antibody | anti-FoxP3 antibody

Anti-FoxP3 Alexa Fluor488

• Gene Names: FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: FoxP3; Monoclonal Antibody; Anti-FoxP3 Alexa Fluor488; Scurfin; JM2; AIID; IPEX; PIDX; XPID; DIETER; forkhead box P3; anti-FoxP3 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: 3G3
• Specificity: The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55kDa transcription factor (intracellular antigen), which is the master regulator in the development and Functional Application (FUNC)tion of regulatory T cells.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-FoxP3 antibody: Flow Cytometry (FC/FACS) (QC tested), Western Blot (WB)
• Application Notes: FC/FACS: Recommended dilution: 1-5ug/ml. Intracellular staining.
• Conjugation: Alexa Fluor488
• Immunogen: Full-length His-tagged recombinant murine FoxP3
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human FoxP3 (3G3) Alexa Fluor 488 antibody (concentration in sample 3 ug/ml).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human CD4 positive lymphocytes using anti-human CD25 (MEM-181) APC antibody (10 ul reagent / 100 ul of peripheral whole blood) and intracellular staining of human CD4 positive lymphocytes using anti-human FoxP3 (3G3) Alexa Fluor 488 antibody (concentration in sample 3 ug/ml).)

Flow Cytometry (FC/FACS)

(Flow cytometry multicolor surface staining of human lymphocytes using anti-human CD4 (MEM-241) Pacific Blue™ antibody (4 ul reagent / 100 ul of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human FoxP3 (3G3) Alexa Fluor 488 antibody (concentration in sample 3 ug/ml).)

Flow Cytometry (FC/FACS)

(Separation of human FoxP3 positive CD25 positive CD4 positive lymphocytes (red-filled) from FoxP3 negative CD25 negative CD4 positive lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human FoxP3 (3G3) Alexa Fluor 488 antibody (concentration in sample 3 ug/ml).)

Related Product Information for anti-FoxP3 antibody

FoxP3 (Forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and Functional Application (FUNC)tion of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysFunctional Application (FUNC)tion, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.

References

*Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry A. 2009 Dec;75(12):1040-50., URL: https://www.ncbi.nlm.nih.gov/pubmed/19845018itool,*Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6659-64., URL: https://www.ncbi.nlm.nih.gov/pubmed/16617117itool
*Kuhn A, Beissert S, Krammer PH: CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus. Arch Dermatol Res. 2009 Jan;301(1):71-81., URL: https://www.ncbi.nlm.nih.gov/pubmed/18985367itool,*Lal G, Bromberg JS: Epigenetic mechanisms of regulation of Foxp3 expression. Blood. 2009 Oct 29;114(18):3727-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/19641188,*Barnes MJ, Powrie F: Regulatory T cells reinforce intestinal homeostasis. Immunity. 2009 Sep 18;31(3):401-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/19766083itool,*Bettini M, Vignali DA: Regulatory T cells and inhibitory cytokines in autoimmunity. Curr Opin Immunol. 2009 Dec;21(6):612-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/19854631itool,*Elkord E: Novel therapeutic strategies by regulatory T cells in allergy. Chem Immunol Allergy. 2008;94:150-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/18802345itool

NCBI and Uniprot Product Information

• NCBI GI #: 167466190
• NCBI GeneID: 50943
• NCBI Accession #: NP_001107849.1
• NCBI GenBank Nucleotide #: NM_001114377.1
• UniProt Accession #: Q9BZS1; O60827; Q14DD8; Q4ZH51; A5HJT1; B7ZLG0
• Molecular Weight: 47,244 Da
• NCBI Official Full Name: forkhead box protein P3 isoform b
• NCBI Official Synonym Full Names: forkhead box P3
• NCBI Official Symbol: FOXP3
• NCBI Official Synonym Symbols: JM2; AIID; IPEX; PIDX; XPID; DIETER
• NCBI Protein Information: forkhead box protein P3; scurfin; FOXP3delta7; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked
• UniProt Protein Name: Forkhead box protein P3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP3
• UniProt Synonym Gene Names: IPEX
• UniProt Entry Name: FOXP3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; C2H2-type zinc finger protein; Cell cycle regulation; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: protein complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; NF-kappaB binding; protein binding; NFAT protein binding; protein homodimerization activity; sequence-specific DNA binding; histone deacetylase binding; metal ion binding; transcription corepressor activity; transcription factor activity

Biological Process: negative regulation of interleukin-10 production; positive regulation of transcription, DNA-dependent; negative regulation of cytokine secretion; myeloid cell homeostasis; T cell receptor signaling pathway; negative regulation of interleukin-2 production; negative regulation of interleukin-6 production; T cell homeostasis; tolerance induction to self antigen; response to virus; negative regulation of transcription factor activity; negative regulation of immune response; cytokine production; negative regulation of T cell cytokine production; positive regulation of peripheral T cell tolerance induction; inhibition of NF-kappaB transcription factor; regulation of T cell anergy; negative regulation of interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; inhibition of CREB transcription factor; negative regulation of transcription, DNA-dependent; negative regulation of cytokine biosynthetic process; transcription from RNA polymerase II promoter; negative regulation of interleukin-4 production; T cell activation; negative regulation of activated T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of histone acetylation; negative regulation of interferon-gamma biosynthetic process; positive regulation of interleukin-4 production; negative regulation of cell proliferation; CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; negative regulation of isotype switching to IgE isotypes; negative regulation of T cell proliferation; B cell homeostasis; regulation of transcription, DNA-dependent; negative regulation of interferon-gamma production; regulation of isotype switching to IgG isotypes; positive regulation of T cell anergy; positive regulation of transforming growth factor-beta1 production; negative regulation of chronic inflammatory response; negative regulation of histone deacetylation; T cell mediated immunity; positive regulation of immature T cell proliferation in the thymus; negative regulation of tumor necrosis factor production; chromatin remodeling; negative regulation of interleukin-17 production; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; negative regulation of interleukin-2 biosynthetic process; positive regulation of histone acetylation

Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked