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Immunohistochemistry (IHC) (Anti-FN1 / Fibronectin antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Mouse anti-Human FN1 / Fibronectin Monoclonal Antibody | anti-FN1 antibody

Anti-FN1 / Fibronectin Antibody (clone 2F4) IHC-plus

Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Ascites
Synonyms
FN1 / Fibronectin; Monoclonal Antibody; Anti-FN1 / Fibronectin Antibody (clone 2F4) IHC-plus; Mouse Monoclonal [clone 2F4] (IgG1) to Human FN1 / Fibronectin; Human FN1 / Fibronectin; CIG; Cold-insoluble globulin; Fibronectin 1; FINC; LETS; GFND; ED-B; Fibronectin; FN; FNZ; GFND2; Migration-stimulating factor; MSF; anti-FN1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2F4
Specificity
Human fibronectin
Purity/Purification
Ascites
Form/Format
Ascites, 0.03% sodium azide
Sequence Length
2355
Applicable Applications for anti-FN1 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
ELISA (1:10000)
Flow Cytometry (1:200 - 1:400)
IHC-P (1:50)
WB (1:500 - 1:2000)
Immunogen
FN1 / Fibronectin antibody was raised against purified recombinant fragment of human FN1 expressed in E Coli.
Immunogen Description
Purified recombinant fragment of human FN1 expressed in E Coli.
Immunogen Type
Recombinant protein
Target Species
Human
Preparation and Storage
Long term: -20 degree C
Short term: 4 degree C
Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-FN1 / Fibronectin antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-FN1 / Fibronectin antibody IHC staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-FN1 / Fibronectin antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC) (Anti-FN1 / Fibronectin antibody IHC staining of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot using FN1 monoclonal antibody against human FN1 (AA: 1965-2176) recombinant protein. (Expected MW is 49.6 kDa))

Western Blot (WB) (Western blot using FN1 monoclonal antibody against human FN1 (AA: 1965-2176) recombinant protein. (Expected MW is 49.6 kDa))

Western Blot (WB)

(Western blot using FN1 monoclonal antibody against HEK293 (1) and FN1 (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.)

Western Blot (WB) (Western blot using FN1 monoclonal antibody against HEK293 (1) and FN1 (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.)

ELISA (EIA)

(Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)

ELISA (EIA) (Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);)
Related Product Information for anti-FN1 antibody
FN1 Antibody, CIG Antibody, Cold-insoluble globulin Antibody, Fibronectin 1 Antibody, FINC Antibody, LETS Antibody, GFND Antibody, ED-B Antibody, Fibronectin Antibody, FN Antibody, FNZ Antibody, GFND2 Antibody, Migration-stimulating factor Antibody, MSF Antibody Description: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
256,502 Da
NCBI Official Full Name
fibronectin isoform 3 preproprotein
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
FN1
NCBI Official Synonym Symbols
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
NCBI Protein Information
fibronectin
UniProt Protein Name
Fibronectin
Protein Family
UniProt Gene Name
FN1
UniProt Synonym Gene Names
FN; FN; CIG
UniProt Entry Name
FINC_HUMAN

NCBI Description

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]

Uniprot Description

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Research Articles on FN1

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Product Notes

The FN1 fn1 (Catalog #AAA249466) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-FN1 / Fibronectin Antibody (clone 2F4) IHC-plus reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FN1 / Fibronectin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA). ELISA (1:10000) Flow Cytometry (1:200 - 1:400) IHC-P (1:50) WB (1:500 - 1:2000). Researchers should empirically determine the suitability of the FN1 fn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FN1 / Fibronectin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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