Mouse anti-Human Fibronectin Monoclonal Antibody | anti-FN1 antibody

Fibronectin (Cell/Heparin2 Binding region) Antibody

Cat. Num. AAA474357

• Gene Names: FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation, Immunocytochemistry
• Purity: Protein G chromatography
• Synonyms: Fibronectin; Monoclonal Antibody; Fibronectin (Cell/Heparin2 Binding region) Antibody; Fibronectin (Cell/Heparin2 Binding region); Fibronectin M013; anti-FN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M013
• Specificity: Clone M013 mouse monoclonal antibody detects a 250kDa* protein on SDS-PAGE immunoblots of native human A549, MeWo, and MDA-MB-231 cells, as well as human plasma fibronectin. The antibody does not detect the denatured form of fibronectin. The antibody also works for immunoprecipitation, immunohistochemistry, and immunocytochemistry, as well as binds fibronectin in live cells and unfixed extracellular matrix.
• Purity/Purification: Protein G chromatography
• Form/Format: Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-FN1 antibody: Western Blot (WB), ELISA (EIA)Cap, Immunoprecipitation (IP), Immunocytochemistry (ICC)
• Application Notes: ELISA (Cap): 1:100; ICC: 1:50; IP: 1:50; WB: 1:1000
• Immunogen: Clone (M013) was generated from a proprietary antigen related to native human fibronectin from the A549 lung carcinoma cell line.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot image of native human cell lysates: A549 (lanes 1 & 5), MeWo (lanes 2 & 6), and MDA-MB-231 (lanes 3 & 7), as well as human plasma fibronectin (lanes 4 & 8). The blot was probed with mouse monoclonal antibodies anti-fibronectin FM0141 (lanes 1-4) or anti-fibronectin FM0131 (lanes 5-8) at 1:1000.)

Immunocytochemistry (ICC)

(Immunocytochemical labeling of fibronectin in paraformaldehyde fixed human A549 cells. The cells were labeled with mouse monoclonal anti-fibronectin (clone M013). The antibody was detected using goat anti-mouse Ig DyLight 594.)

Related Product Information for anti-FN1 antibody

In the extracellular matrix, fibronectin provides essential connections to cells through interaction with integrins and other receptors that regulate cell adhesion, migration, and differentiation. Fibronectin is secreted as a large dimeric glycoprotein with subunits that range in size from 230kDa to 270kDa. Fibronectin is composed of three different types of modules termed type I, II, and III repeats, as well as two fibrin binding and two heparin binding domains, a collagen interaction region and cell attachment domain. The diverse set of binding domains provides fibronectin with the ability to interact simultaneously with other fibronectin molecules, other ECM components (e.g., collagens and proteoglycans), cell surface receptors, and extracellular enzymes. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, while cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Fibronectin fibrilogenesis has important functions during tissue development, and during pathological progression of tissues and wound healing.

References

Zollinger AJ & Smith ML (2017) Matrix Biol. 60-61:27.
Wang Y & Ni H. (2016) Cell Mol Life Sci. 73(17):3265.
Schwarzbauer J & DeSimone D (2011) CSH Persp Biol. 1;3(7).

NCBI and Uniprot Product Information

• NCBI GI #: 16933542
• NCBI GeneID: 2335
• NCBI Accession #: NP_002017.1
• NCBI GenBank Nucleotide #: NM_002026.2
• UniProt Accession #: P02751; O95609; O95610; Q14312; Q14325; Q14326; Q17RV7; Q585T2; Q59EH1; Q60FE4; Q68DP8; Q68DP9
• Molecular Weight: 262,625 Da
• NCBI Official Full Name: fibronectin isoform 3 preproprotein
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: FN1
• NCBI Official Synonym Symbols: FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
• NCBI Protein Information: fibronectin; cold-insoluble globulin; migration-stimulating factor
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: FN1
• UniProt Synonym Gene Names: FN; FN; CIG
• UniProt Entry Name: FINC_HUMAN

NCBI Description

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: extracellular matrix; extracellular space; apical plasma membrane; fibrinogen complex; extracellular region; ER-Golgi intermediate compartment; basal lamina

Molecular Function: heparin binding; collagen binding; integrin binding; protein binding; protease activator activity; protease binding

Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; extracellular matrix disassembly; regulation of cell shape; platelet degranulation; acute-phase response; calcium-independent cell-matrix adhesion; cell-substrate junction assembly; response to wounding; peptide cross-linking; angiogenesis; cell adhesion; blood coagulation; leukocyte migration

Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency