Mouse Fibrillarin Monoclonal Antibody | anti-FBL antibody

Fibrillarin (FBL, 34kD Nucleolar Scleroderma Antigen, rRNA 2'-O-methyltransferase fibrillarin, FLRN, FIB1, Nop1p, RNU3IP1)

Cat. Num. AAA608427

• Gene Names: FBL; FIB; FLRN; RNU3IP1
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Fibrillarin; Monoclonal Antibody; Fibrillarin (FBL; 34kD Nucleolar Scleroderma Antigen; rRNA 2'-O-methyltransferase fibrillarin; FLRN; FIB1; Nop1p; RNU3IP1); Anti -Fibrillarin (FBL; anti-FBL antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C147
• Specificity: Recognizes human Fibrillarin.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
• Concentration: 0.1 mg/mL (varies by lot)

• Applicable Applications for anti-FBL antibody: Western Blot (WB). Other applications not tested.
• Application Notes: Optimal dilutions to be determined by the researcher.
• Immunogen: Partial sequence of recombinant full-length protein to human Fibrillarin
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior removing the cap.

Testing Data

Related Product Information for anti-FBL antibody

Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Product Categories/Family for anti-FBL antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 31395
• NCBI GeneID: 2091
• UniProt Accession #: P22087; O75259; Q6IAT5; Q9UPI6; B5BUE8; Q96BS4
• NCBI Official Full Name: fibrillarin
• NCBI Official Synonym Full Names: fibrillarin
• NCBI Official Symbol: FBL
• NCBI Official Synonym Symbols: FIB; FLRN; RNU3IP1
• NCBI Protein Information: rRNA 2'-O-methyltransferase fibrillarin; 34-kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; RNA, U3 small nucleolar interacting protein 1
• UniProt Protein Name: rRNA 2'-O-methyltransferase fibrillarin
• Protein Family: Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase
• UniProt Gene Name: FBL
• UniProt Synonym Gene Names: FIB1; FLRN
• UniProt Entry Name: FBRL_HUMAN

NCBI Description

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq]

Uniprot Description

FBL: Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'- hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Interacts with NOLC1. Component of box C/D small nucleolar ribonucleoprotein (snoRNP) particles that contain NHP2L1, FBL, NOP5 and NOP56, plus a guide RNA. It is associated with the U3, U8, U13, X and Y small nuclear RNAs. Component of several ribosomal and nucleolar protein complexes. Interacts with PRMT5 and UTP20. Interacts with DDX5. Belongs to the methyltransferase superfamily. Fibrillarin family.

Protein type: EC 2.1.1.-; RNA-binding; Nucleolus; Methyltransferase; RNA processing

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: Cajal body; granular component; membrane; nucleolus; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: osteoblast differentiation; snoRNA metabolic process; tRNA processing; rRNA processing