Mouse anti-Human FGFR4 Monoclonal Antibody | anti-FGFR4 antibody

FGFR4 (Fibroblast Growth Factor Receptor 4, FGFR-4, CD334 Antigen, FGFR4, JTK2, TKF) (MaxLight 405)

Cat. Num. AAA6188360

• Gene Names: FGFR4; TKF; JTK2; CD334
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: FGFR4; Monoclonal Antibody; FGFR4 (Fibroblast Growth Factor Receptor 4; FGFR-4; CD334 Antigen; JTK2; TKF) (MaxLight 405); anti-FGFR4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10i43
• Specificity: Recognizes human FGFR4.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-FGFR4 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Purified recombinant protein corresponding to human FGFR4 fusion protein
• Conjugate: MaxLight405
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of FGFR4 recombinant protein (fragment 42kD) using. FGFR4 protein (arrow) was detected using the purified Mab.)

Western Blot (WB)

(Western Blot analysis in 293 cell lysates (2ug/lane) using either nontransfected (Lane 1) or transiently transfected with the FGFR4 gene (Lane 2).)

Related Product Information for anti-FGFR4 antibody

FGFR4 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.

Product Categories/Family for anti-FGFR4 antibody

Antibodies; Receptors

References

1. Jezequel, P., et al., Br. J. Cancer 90(1):189-193 (2004). 2.Qian, Z.R., et al., J. Clin. Endocrinol. Metab. 89(4):1904-1911 (2004). 3.Yu, S., et al., J. Biol. Chem. 278(22):19597-19602 (2003). 4.Becker, N., et al., Cancer Epidemiol. Biomarkers Prev. 12(6):582-583 (2003). 5.Takenaka, H., et al., Arch. Dermatol. Res. 294(7):331-338 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 47524173
• NCBI GeneID: 2264
• NCBI Accession #: NP_002002
• NCBI GenBank Nucleotide #: NM_002011.4
• UniProt Accession #: P22455; O43785; Q14309; Q71TW8; Q8TDA0; Q96KE5; G3JVM2; G3JVM5; G3JVM7; G3JVM9
• Molecular Weight: 39.5kDa (356aa) 40-57kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: fibroblast growth factor receptor 4 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 4
• NCBI Official Symbol: FGFR4
• NCBI Official Synonym Symbols: TKF; JTK2; CD334
• NCBI Protein Information: fibroblast growth factor receptor 4
• UniProt Protein Name: Fibroblast growth factor receptor 4
• Protein Family: Fibroblast growth factor receptor
• UniProt Gene Name: FGFR4
• UniProt Synonym Gene Names: JTK2; TKF; FGFR-4
• UniProt Entry Name: FGFR4_HUMAN

NCBI Description

The protein encoded by this gene is a tyrosine kinase and cell surface receptor for fibroblast growth factors. The encoded protein is involved in the regulation of several pathways, including cell proliferation, cell differentiation, cell migration, lipid metabolism, bile acid biosynthesis, vitamin D metabolism, glucose uptake, and phosphate homeostasis. This protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. [provided by RefSeq, Aug 2017]

Uniprot Description

FGFR4: a tyrosine-kinase receptor that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of the MAP kinase and AKT signaling pathways. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling. Two isoforms of the human protein are formed by alternative splicing. This description may include information from UniProtKB

Protein type: Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; TK group; FGFR family

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: nucleoplasm; Golgi apparatus; transport vesicle; endoplasmic reticulum; integral to plasma membrane; cytoplasm; plasma membrane; extracellular region; intercellular junction; endosome

Molecular Function: heparin binding; protein binding; fibroblast growth factor binding; fibroblast growth factor receptor activity; protein-tyrosine kinase activity; ATP binding

Biological Process: epidermal growth factor receptor signaling pathway; cell migration; fibroblast growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; regulation of lipid metabolic process; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of proteolysis; protein amino acid autophosphorylation; positive regulation of metalloenzyme activity; glucose homeostasis; induction of an organ; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; phosphate ion homeostasis