Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGFA/FHFA (pan) protein using Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody, Clone S235-22. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.)
Mouse FGFA/FHFA (pan) Monoclonal Antibody | anti-FGF13 antibody
FGFA/FHFA (pan) Antibody
Shipping Temperature: Blue Ice or 4ºC
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGFA/FHFA (pan) protein using Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody, Clone S235-22. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of ~30 kDa FGFA/FHFA (pan) protein using Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody, Clone S235-22. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-FGFA/FHFA (pan) Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~30 kDa.)
Scientific Background: FGF13(Fibroblast growth factor 13), also called FHF2 is a protein that in humans is encoded by the FGF13 gene.The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF13is a large gene, extending over approximately 200 kb in Xq26.3, and contains at least 7 exons. By cytogenetic, FISH, and database analysis, Gecz et al. (1999) localized the FGF13 gene within a 400-kb duplication interval on chromosome Xq26.3. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This gene is located to a region associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a syndromal X-linked mental retardation, which suggests it may be a candidate gene for familial cases of the BFL syndrome. The function of this gene has not yet been determined. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This gene is located in a region on chromosome X, which is associated with Borjeson-Forssman-Lehmann syndrome (BFLS), making it a possible candidate gene for familial cases of the BFLS, and for other syndromal and nonspecific forms of X-linked mental retardation mapping to this region. Alternative splicing of this gene at the 5' end results in several transcript variants encoding different isoforms with different N-termini. [provided by RefSeq, Nov 2008]
Uniprot Description
FGF13: Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules. Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching. Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus. Belongs to the heparin-binding growth factors family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; Nucleolus
Chromosomal Location of Human Ortholog: Xq26.3
Cellular Component: microtubule; neuron projection; growth cone; axon; cytoplasm; dendrite; extracellular region; plasma membrane; nucleolus; nucleus; filopodium
Molecular Function: protein binding; growth factor activity; sodium channel regulator activity; microtubule binding; beta-tubulin binding; protein kinase activator activity
Biological Process: nervous system development; negative regulation of microtubule depolymerization; establishment of neuroblast polarity; hippocampus development; MAPKKK cascade; neuron migration; learning; negative regulation of collateral sprouting; signal transduction; memory; cerebral cortex cell migration; cell-cell signaling; positive regulation of protein kinase activity; microtubule polymerization
Research Articles on FGF13
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Product Notes
The FGF13 fgf13 (Catalog #AAA801124) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FGFA/FHFA (pan) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FGFA/FHFA (pan) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF). WB (1:1000); optimal dilutions for assays should be determined by the user. Researchers should empirically determine the suitability of the FGF13 fgf13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGFA/FHFA (pan), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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