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Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant FGF21 protein (red) or empty vector (blue) stained using FGF21 antibody)

Mouse FGF21 Monoclonal Antibody | anti-FGF21 antibody

FGF21 antibody

Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
FGF21 antibody was purified by affinity chromatography.
Synonyms
FGF21; Monoclonal Antibody; FGF21 antibody; Monoclonal FGF21; Anti-FGF21; fibroblast growth factor 21; FGF 21; FGF-21; anti-FGF21 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2F10
Purity/Purification
FGF21 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Sequence Length
209
Applicable Applications for anti-FGF21 antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
FC: 1:100
IHC: 1:150
WB: 1:2000
Biological Significance
FGF21 is a secreted growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors and stimulates insulin independent glucose uptake by adipocytes.
Cross-Reactivity
Human
Immunogen
FGF21 antibody was raised in Mouse using Human recombinant protein fragment corresponding to amino acids 29-209 of human FGF21 produced in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant FGF21 protein (red) or empty vector (blue) stained using FGF21 antibody)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant FGF21 protein (red) or empty vector (blue) stained using FGF21 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FGF21 protein (Right) or empty vector (Left) detected with FGF21 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant FGF21 protein (Right) or empty vector (Left) detected with FGF21 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of FGF21 protein in paraffin embedded Carcinoma of Human liver tissue using FGF21 antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of FGF21 protein in paraffin embedded Carcinoma of Human liver tissue using FGF21 antibody)
Related Product Information for anti-FGF21 antibody
Mouse monoclonal FGF21 antibody
Product Categories/Family for anti-FGF21 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,300 Da
NCBI Official Full Name
fibroblast growth factor 21
NCBI Official Synonym Full Names
fibroblast growth factor 21
NCBI Official Symbol
FGF21
NCBI Protein Information
fibroblast growth factor 21
UniProt Protein Name
Fibroblast growth factor 21
Protein Family
UniProt Gene Name
FGF21
UniProt Synonym Gene Names
FGF-21
UniProt Entry Name
FGF21_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: positive regulation of glucose import; cell-cell signaling; positive regulation of cell proliferation; positive regulation of low-density lipoprotein receptor biosynthetic process; signal transduction

Research Articles on FGF21

Similar Products

Product Notes

The FGF21 fgf21 (Catalog #AAA837991) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FGF21 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). FC: 1:100 IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the FGF21 fgf21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGF21, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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