Mouse anti-Human FCER1A Monoclonal Antibody | anti-FCER1A antibody

FCER1A (High Affinity Immunoglobulin epsilon Receptor Subunit alpha, Fc-epsilon RI-alpha, FcERI, IgE Fc Receptor Subunit alpha, FCE1A) (AP)

Cat. Num. AAA6131273

• Gene Names: FCER1A; FCE1A; FcERI
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FCER1A; Monoclonal Antibody; FCER1A (High Affinity Immunoglobulin epsilon Receptor Subunit alpha; Fc-epsilon RI-alpha; FcERI; IgE Fc Receptor Subunit alpha; FCE1A) (AP); anti-FCER1A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2C12-3B6
• Specificity: Recognizes human FCER1A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-FCER1A antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-257 from FCER1A (AAH05912.1) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAPAMESPTLLCVALLFFAPDGVLAVPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSLSEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDVYKVIYYKDGEALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPREKYWLQFFIPLLVVILFAVDTGLFISTQQQVTFLLKIKRTRKGFRLLNPHPKPNPKNN
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (54.01kD).)

Western Blot (WB)

(FCER1A monoclonal antibody, Western Blot analysis of FCER1A expression in A-549.)

Western Blot (WB)

(Western Blot analysis of FCER1A expression in transfected 293T cell line by FCER1A monoclonal antibody. Lane 1: FCER1A transfected lysate (29.6kD). Lane 2: Non-transfected lysate.)

Western Blot (WB)

(Western blot analysis of FCER1A over-expressed 293 cell line, cotransfected with FCER1A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FCER1A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Related Product Information for anti-FCER1A antibody

High affinity IgE receptor (FceRI) plays a key role in IgE-mediated allergic immune response. FceRI is a tetrameric receptor complex, which is composed of one a-subunit (FceRIa), one B-subunit, and two y-subunits. FceRIa directly binds IgE with high affinity, while the B- and y-chains are responsible for mediating intracellular signals. FceRIa is a 50kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FceRIa is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.

Product Categories/Family for anti-FCER1A antibody

Antibodies; Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 13543505
• NCBI GeneID: 2205
• Molecular Weight: 29,596 Da
• NCBI Official Full Name: Homo sapiens Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide, mRNA
• NCBI Official Synonym Full Names: Fc fragment of IgE receptor Ia
• NCBI Official Symbol: FCER1A
• NCBI Official Synonym Symbols: FCE1A; FcERI
• NCBI Protein Information: high affinity immunoglobulin epsilon receptor subunit alpha
• Protein Family: High affinity immunoglobulin epsilon receptor

NCBI Description

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]