Western Blot (WB)
(Western Blot: Sample: Rabbit Serum; Primary Ab: 5ug/ml Cavia Anti-Rabbit MMP13 Antibody Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody )
Guinea Pig anti-Rabbit Matrix Metalloproteinase 13 (MMP13) Polyclonal Antibody | anti-MMP13 antibody
Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13)
Reactivity
Rabbit
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Matrix Metalloproteinase 13 (MMP13); Polyclonal Antibody; Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13); anti-MMP13 antibody
Host
Guinea Pig
Reactivity
Rabbit
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MMP13. It has been selected for its ability to recognize MMP13 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGS- E MQSFFGLEVT GKLDDNTLAI MKQPRCGVPD VGEYNVFPRT LKWSQTNLTY RIVNYTPDLT HSEVEKAFKK AFKVWSDVTP LNFTRIHNGT ADIMISFGTK EHGDFYPFDG PSGLLAHAFP PGPNYGGDAH FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYT
MGHHHHHHSGS- E MQSFFGLEVT GKLDDNTLAI MKQPRCGVPD VGEYNVFPRT LKWSQTNLTY RIVNYTPDLT HSEVEKAFKK AFKVWSDVTP LNFTRIHNGT ADIMISFGTK EHGDFYPFDG PSGLLAHAFP PGPNYGGDAH FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYT
Sequence Length
471
Applicable Applications for anti-MMP13 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant MMP13 (Glu70~Thr245) expressed in E.coli.
Cross Reactivity
Rabbit
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rabbit Serum; Primary Ab: 5ug/ml Cavia Anti-Rabbit MMP13 Antibody Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rabbit Serum; Primary Ab: 5ug/ml Cavia Anti-Rabbit MMP13 Antibody Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Cavia IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,693 Da
NCBI Official Full Name
collagenase 3
NCBI Official Symbol
MMP13
NCBI Protein Information
collagenase 3
UniProt Protein Name
Collagenase 3
Protein Family
UniProt Gene Name
MMP13
UniProt Synonym Gene Names
MMP-13
Uniprot Description
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion ().
Research Articles on MMP13
Similar Products
Product Notes
The MMP13 mmp13 (Catalog #AAA2007133) is an Antibody produced from Guinea Pig and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Matrix Metalloproteinase 13 (MMP13) reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Matrix Metalloproteinase 13 (MMP13) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MMP13 mmp13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG S- E MQSFFGLEVT GKLDDNTLAI MKQPRCGVPD VGEYNVFPRT LKWSQTNLTY RIVNYTPDLT HSEVEKAFKK AFKVWSDVTP LNFTRIHNGT ADIMISFGTK EHGDFYPFDG PSGLLAHAFP PGPNYGGDAH FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYT. It is sometimes possible for the material contained within the vial of "Matrix Metalloproteinase 13 (MMP13), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
