Mouse anti-Human Fas Ligand (CD178) Monoclonal Antibody | anti-FASLG antibody

Mouse Anti-Human Fas Ligand-UNLB

Cat. Num. AAA670167

• Gene Names: FASLG; FASL; CD178; CD95L; CD95-L; TNFSF6; APT1LG1
• Reactivity: Human
• Applications: Flow Cytometry, Immunoprecipitation, Western Blot
• Synonyms: Fas Ligand (CD178); Monoclonal Antibody; Mouse Anti-Human Fas Ligand-UNLB; Mouse Anti-Human Fas Ligand - Purified; Unlabeled Antibody; Mouse anti-Human Fas Ligand (CD178); anti-FASLG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: SB93a1
• Specificity: Reacts with native and recombinant human Fas Ligand1; Fas Ligand (CD178) is a 40 kD type II transmembrane protein that is a member of the tumor necrosis factor superfamily. This well characterized potent apoptotic factor is utilized by cytotoxic T cells and natural killer cells to selectively kill virus infected and tumorigenic cells2,3. Moreover, studies indicate that Fas Ligand is an important regulator in immune homeostasis where it has been shown to down-regulate immune responses during activation-induced cell death4. Fas Ligand initiates apoptosis by binding to its receptor (CD95) on target cells which facilitates recruitment of numerous signaling proteins to form a death inducing signaling complex. This signaling complex is then believed to propagate the apoptotic signal through the recruitment and activation of Caspase- 85.; The SB93a monoclonal antibody detects a band at ~40 kD, corresponding to the transmembrane (insoluble) form of Fas Ligand.
• Form/Format: Purified (UNLB) Antibody
• Sequence Length: 281

• Applicable Applications for anti-FASLG antibody: Flow Cytometry; Immunoprecipitation; Western Blot
• Application Notes: Flow Cytometry Suggested Dilution: <= 1 uL/10^6 cells; Western Blot Suggested Dilution: 0.5-2 ug; Immunoprecipitation Suggested Dilution: 5-20 ug
• Immunogen: Recombinant human Fas Ligand
• Characterization: To ensure acceptable performance, each batch of product is tested by Flow Cytometry and/or Western blotting to conform to characteristics of a standard reference reagent.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.1mg purified immunoglobulin in 0.2mL of 100 mM borate buffered saline, pH 8.0; store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2- 8 degree C. ; Reagents are stable for the period shown on the label if stored as directed.

References

1) Trambas, C.M. and Griffiths, G.M. (2003) Nat. Immunol. 4:399-403.
3) Voss, M. et al. (2008). Cell. Commun. Signal. 6:11.
4) Krammer, P.H. (2000). Nature. 407:789-95..
5) Peter, M.E. and Krammer, P.H. (2002). Cell. Death Diff. 10:26-35.

NCBI and Uniprot Product Information

• NCBI GI #: 17028381
• NCBI GeneID: 356
• UniProt Accession #: P48023; Q9BZP9; Q0VHD7; Q53ZZ1
• Molecular Weight: 31,485 Da
• NCBI Official Full Name: Fas ligand (TNF superfamily, member 6)
• NCBI Official Synonym Full Names: Fas ligand (TNF superfamily, member 6)
• NCBI Official Symbol: FASLG
• NCBI Official Synonym Symbols: FASL; CD178; CD95L; CD95-L; TNFSF6; APT1LG1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6; APTL; CD95 ligand; OTTHUMP00000032708; fas antigen ligand; apoptosis antigen ligand; apoptosis (APO-1) antigen ligand 1; tumor necrosis factor (ligand) superfamily, member 6
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 6
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: FASLG
• UniProt Synonym Gene Names: APT1LG1; CD95L; FASL; TNFSF6
• UniProt Entry Name: TNFL6_HUMAN

NCBI Description

The protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with this ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). [provided by RefSeq]

Uniprot Description

Function: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.

Subunit structure: Homotrimer

Probable. Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Ref.12

Subcellular location: Cell membrane; Single-pass type II membrane protein. Secreted. Note: May be released into the extracellular fluid, probably by cleavage form the cell surface.

Post-translational modification: N-glycosylated.The soluble form derives from the membrane form by proteolytic processing.

Involvement in disease: Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [

MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. Ref.9

Sequence similarities: Belongs to the tumor necrosis factor family.