Mouse anti-Human FADD Monoclonal Antibody | anti-FADD antibody

FADD antibody

Cat. Num. AAA200102

• Gene Names: FADD; GIG3; MORT1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: By protein-G affinity chromatography
• Synonyms: FADD; Monoclonal Antibody; FADD antibody; Fas-associated via death domain; GIG3; MORT1; MGC8528; Fas-associated via death domain FADD protein; Fas TNFRSF6 associated via death domain; Fas (TNFRSF6) associated via death domain; Fas associated via death domain; Fas associating protein; Fas associating death domain containing protein; Fas associating protein with death domain GIG 3; Growth inhibiting gene 3 protein; H sapiens mRNA for mediator of receptor induced toxicity; Mediator of receptor induced toxicity; MORT 1.; anti-FADD antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, k
• Clone Number: J1D2
• Purity/Purification: By protein-G affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 208

• Applicable Applications for anti-FADD antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100.; Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.
• Antigen Species: Human
• Immunogen: Recombinant human FADD (1-208aa) purified from E. coli
• Clone Note: Anti-human FADD mAb, clone J1D2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FADD protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(Cell lysates of Jurkat (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FADD (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FADD antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Raw264.7 cell lysate Lane 3. : MCF7 cell lysate Lane 4. : A431 cell lysate Lane 5. : Ramos cell lysate Lane 6. : Raji cell lysate Lane 7. : Balb/3T3 cell lysate)

Immunohistochemistry (IHC)

(Paraffin embedded sections of human breast cancer tissue were incubated with anti-human FADD (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))

Immunofluorescence (IF)

(ICC/IF analysis of FADD in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human FADD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Related Product Information for anti-FADD antibody

FADD (Fas-associated protein with death domain) is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. This protein is implicated in survival/proliferation and cell cycle progression. FADD functions are also regulated via cellular sublocalization, protein phosphorylation, and inhibitory molecules.

Product Categories/Family for anti-FADD antibody

Apoptosis

References

Lea Tourneur.,et al: (2005) Medical Immunology. 4:1. Tsao,C.H., et al: (2008) J. Gen. Virol. 89; (PT 8), 1930-1941.; Douglas D. Bannerman, et al. (2002). J. Clin. Invest. 109:419-425.

NCBI and Uniprot Product Information

• NCBI GI #: 4505229
• NCBI GeneID: 8772
• NCBI Accession #: NP_003815
• NCBI GenBank Nucleotide #: NM_003824.3
• UniProt Accession #: Q13158; Q14866; Q6IBR4
• Molecular Weight: 23,279 Da
• NCBI Official Full Name: FAS-associated death domain protein
• NCBI Official Synonym Full Names: Fas associated via death domain
• NCBI Official Symbol: FADD
• NCBI Official Synonym Symbols: GIG3; MORT1
• NCBI Protein Information: FAS-associated death domain protein
• UniProt Protein Name: FAS-associated death domain protein
• Protein Family: Fas-associated death domain protein
• UniProt Gene Name: FADD
• UniProt Synonym Gene Names: MORT1

NCBI Description

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]

Uniprot Description

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.