Mouse anti-Human FADD Monoclonal Antibody | anti-FADD antibody

Anti-FADD Mouse mAb

Cat. Num. AAA475356

• Gene Names: FADD; GIG3; MORT1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: FADD; Monoclonal Antibody; Anti-FADD Mouse mAb; FADD protein; FADD_HUMAN; Fas (TNFRSF6) associated via death domain; Fas associated via death domain; Fas associating death domain containing protein; Fas associating protein; Fas associating protein with death domain; Fas TNFRSF6 associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; FasTNFRSF6 associated via death domain; GIG 3; GIG3; Growth inhibiting gene 3 protein; Growth-inhibiting gene 3 protein; H sapiens mRNA for mediator of receptor induced toxicity; Mediator of receptor induced toxicity; MGC8528; MORT 1; MORT1; Protein FADD; anti-FADD antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 5A3-C7-G9
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
• Sequence Length: 208

• Applicable Applications for anti-FADD antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500
• Immunogen: Recombinant human FADD protein.
• Sensitivity: This antibody detects endogenous levels of FADD and does not cross-react with related proteins.
• Sequence Similarities: Contains 1 death domain.Contains 1 DED (death effector) domain.
• Subunit Structure: Can self-associate. Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and PIDD1. Interacts (via death domain) with FAS (via death domain). Interacts with CASP8. Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation (By similarity).

Western Blot (WB)

(Western blot detection of FADD in SW480, Jurkat and Hela cell lysates using FADD mouse mAb (dilution 1:500).Predicted band size:28kDa.Observed band size:28kDa.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using FADD mouse mAb.)

Related Product Information for anti-FADD antibody

Entrez Summary: The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals.Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors.Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade.Knockout studies in mice also suggest the importance of this protein in early T cell development.[provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 4505229
• NCBI GeneID: 8772
• NCBI Accession #: NP_003815.1
• NCBI GenBank Nucleotide #: NM_003824
• UniProt Accession #: Q13158; Q14866; Q6IBR4
• Molecular Weight: 28
• NCBI Official Full Name: FAS-associated death domain protein
• NCBI Official Synonym Full Names: Fas associated via death domain
• NCBI Official Symbol: FADD
• NCBI Official Synonym Symbols: GIG3; MORT1
• NCBI Protein Information: FAS-associated death domain protein
• UniProt Protein Name: FAS-associated death domain protein
• Protein Family: Fas-associated death domain protein
• UniProt Gene Name: FADD
• UniProt Synonym Gene Names: MORT1

NCBI Description

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]

Uniprot Description

FADD: an adaptor molecule that mediates apoptosis. Recruited through its C-terminal death domain by Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TRAIL-receptor, participating in the death signaling initiated by these receptors. Interaction with the receptors unmasks the N-terminal effector domain of this protein, which recruits caspase-8, and thereby activates the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: CD95 death-inducing signaling complex; cytoplasm; cytosol; plasma membrane

Molecular Function: identical protein binding; protease binding; protein binding; tumor necrosis factor receptor superfamily binding

Biological Process: apoptosis; caspase activation; cell surface receptor linked signal transduction; defense response to virus; induction of apoptosis via death domain receptors; lymph node development; positive regulation of activated T cell proliferation; positive regulation of adaptive immune response; positive regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon-gamma production; positive regulation of interleukin-8 production; positive regulation of macrophage differentiation; positive regulation of proteolysis; positive regulation of T cell mediated cytotoxicity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; spleen development; T cell differentiation in the thymus; T cell homeostasis; thymus development

Disease: Infections, Recurrent, With Encephalopathy, Hepatic Dysfunction, And Cardiovascular Malformations