Plasminogen ELISA Kit | PLG elisa kit

Cyno monkey plasminogen total antigen assay ELISA kit

Cat. Num. AAA136009

• Synonyms: Plasminogen; Cyno monkey plasminogen total antigen assay ELISA kit; PLG elisa kit

• Sequence Length: 810

• Shipping Note: This product cannot be shipped outside of USA.
• Preparation and Storage: Store at 4 degree C

Testing Data

Related Product Information for PLG elisa kit

Plasminogen is a single chain glycoprotein zymogen and is the precursor of the fibrinolytic enzyme plasmin. Plasminogen deficiencies are classified as hypoplasminogenemia (Type I) or dysplasminogenemia (Type 2) and are associated with decreased extracellular fibrin clearance leading to mucous membrane lesions and ligneous conjunctivitis. The sensitive quantitative measurement of total cynomolgus macaque monkey (Macaca fascicularis) plasminogen antigen in plasma, serum, or cell culture media samples is easily performed with this 96 well strip format ELISA kit. Plasminogen, plasmin and plasmin antiplasmin complex will be detected by the assay. The concentration of plasminogen in normal human plasma is 195 ug/ml. Normal values of plasminogen in cyno plasma have not been conclusively determined but are believed to be similar to human plasma. The assay measures total cyno plasminogen in the 0.5-100 ng/ml range. Samples giving cyno plasminogen levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:10,000-1:100,000 dilution for plasma is suggested for best results. Cyno plasminogen will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-cyno plasminogen primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of plasminogen in the samples. A standard calibration curve is prepared using dilutions of purified plasminogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Product Categories/Family for PLG elisa kit

PLASMINOGEN ELISA Kits; Monkey Kits; Plasminogen ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 190026
• NCBI GeneID: 5340
• Molecular Weight: 90,569 Da
• NCBI Official Full Name: plasminogen
• NCBI Official Synonym Full Names: plasminogen
• NCBI Official Symbol: PLG
• NCBI Protein Information: plasminogen
• UniProt Protein Name: Plasminogen
• Protein Family: Plasminogen
• UniProt Gene Name: PLG
• UniProt Entry Name: PLMN_HUMAN

Uniprot Description

Plasminogen: Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. Defects in PLG are the cause of plasminogen deficiency (PLGD). PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. Belongs to the peptidase S1 family. Plasminogen subfamily.

Protein type: EC 3.4.21.7; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: cell surface; extracellular region; extracellular space; extrinsic to external side of plasma membrane; plasma membrane

Molecular Function: apolipoprotein binding; protein binding; protein domain specific binding; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: blood coagulation; cellular protein metabolic process; extracellular matrix disassembly; fibrinolysis; negative regulation of cell proliferation; negative regulation of fibrinolysis; platelet degranulation; positive regulation of fibrinolysis

Disease: Plasminogen Deficiency, Type I