Mouse anti-E Coli Extracellular Death Factor Monoclonal Antibody | anti-zwf antibody

Anti Extracellular Death Factor mouse monoclonal antibody

Cat. Num. AAA157892

• Gene Names: zwf; ECK1853; JW1841
• Reactivity: E Coli
• Applications: ELISA, Immunohistochemistry
• Purity: Protein G purified from mice ascites
• Synonyms: Extracellular Death Factor; Monoclonal Antibody; Anti Extracellular Death Factor mouse monoclonal antibody; Description: mazEF is a toxin-antitoxin stress-induced module which is abundant on the chromosome of most bacteria including pathogens and most extensively studied in Escherichia coli. E Coli mazEF mediated cell death is a population phenomenon requiring the quorum-sensing (QS) -Extracellular Death Factor' (EDF) ; the E Coli peptide NNWNN. Penta-peptide NNWNN (EcEDF) enhances the endoribonucleolytic activity of E Coli toxin MazF. The newly discovered extracellular death factor (EDF) is a pentapeptide with the sequence NNWNN in Escherichia coli. It was reported that it participated in the cell death process mediated by toxin-antitoxin system mazEF. Reactive oxygen species (ROS) are recently considered as common factors for bactericidal antibiotics-mediated cell death. Previous study indicated that EDF could scavenge hydroxyl radicals and might act as a signal molecule with dual effects; œdeath and œsurvival. But the structure-activity relationship of EDF and the effects of EDF on the activity of antibiotics remain unclear. In the present study; our results indicated that tryptophan could be the key residue to the hydroxyl radicals-scavenging activity of EDF; and EDF could protect Escherichia coli from killing by bactericidal antibiotics; but not by DNA-damaging or bacteriostatic antibiotics. Our results could provide novel evidence to understand the role of EDF in drug-resistance.; anti-zwf antibody

• Host: Mouse
• Reactivity: E Coli
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 93-D-4
• Purity/Purification: Protein G purified from mice ascites
• Form/Format: Liquid. Supplied as solution form in PBS, pH7.4, containing 0.05% proclin300, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-zwf antibody: Elisa (EIA), Immunohistochemistry (IHC)
• Application Notes: Elisa: 1:1000~5000; IHC: 1:50~100
• Immunogen: NNWNN
• Conjugation: Unconjugate
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4 degree C for frequent use. Store at -20 to -80 degree C for ~12 months from the date of receipt

Product Categories/Family for anti-zwf antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 16129805
• NCBI GeneID: 946370
• NCBI Accession #: NP_416366
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0AC53; P22992; P78069; Q60134; Q60139
• Molecular Weight: 55.7kDa (491 aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: glucose-6-phosphate 1-dehydrogenase
• NCBI Official Symbol: zwf
• NCBI Official Synonym Symbols: ECK1853; JW1841
• NCBI Protein Information: glucose-6-phosphate 1-dehydrogenase
• UniProt Protein Name: Glucose-6-phosphate 1-dehydrogenase
• Protein Family: Glucose-6-phosphate 1-dehydrogenase
• UniProt Gene Name: zwf
• UniProt Synonym Gene Names: EDF

NCBI Description

Residues 199-203 of zwf encode a NNWDN peptide involved in ChpA(MazF) toxicity that is likely converted to NNWNN by AsnA; a zwf deletion prevents NNWNN activity (Kolodkin-Gal, 2007). [More information is available at EcoGene: EG11221]. A null mutation in the zwf gene encoding glucose 6-phosphate-1-dehydrogenase does not affect the growth rate significantly. [More information is available at EcoCyc: EG11221].

Uniprot Description

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.