Mouse Eukaryotic Translation Initiation Factor 5A Monoclonal Antibody | anti-EIF5A antibody

Mouse Anti Human Eukaryotic Translation Initiation Factor 5A

Cat. Num. AAA140347

• Gene Names: EIF5A; EIF-5A; EIF5A1; eIF5AI
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: EIF5A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Eukaryotic Translation Initiation Factor 5A; Monoclonal Antibody; Mouse Anti Human Eukaryotic Translation Initiation Factor 5A; EIF5A Antibody; Mouse Anti Human; EIF-5A; EIF5A1; eIF5AI; MGC99547; MGC104255; EIF5A; Eukaryotic translation initiation factor 5A-1; eIF-5A-1; eIF-5A1; Eukaryotic initiation factor 5A isoform 1; eIF-4D; Rev-binding factor; anti-EIF5A antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain and kappa light chain
• Clone Number: PAT2E9AT
• Purity/Purification: EIF5A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 184

• Applicable Applications for anti-EIF5A antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: EIF5A antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500.Recommended starting dilution is 1:250.
• Immunogen: Anti-human EIF5A mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human EIF5A amino acids 1-154 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-EIF5A antibody

Introduction: EIF5A is the single protein identified to contain remarkable amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. EIF5A takes part in the first step of peptide bond formation in translation, nevertheless further experiments implicates it as a universally conserved translation elongation factor. Modulation of EIF5A is connected to proliferation and cancer. Expression of EIF-5A is upregulated in the PBMCs of HIV-1 patients. EIF5A coordinates significant cellular processes like cell viability and senescence during its effects on the stability of certain mRNAs. Heat stress-induced loss of EIF-5A in a human pancreatic cancer cell line. EIF5A stability takes part in determining the fate of the particular cell type after severe heat stress.

Product Categories/Family for anti-EIF5A antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 219555707
• NCBI GeneID: 1984
• NCBI Accession #: NP_001137232.1
• NCBI GenBank Nucleotide #: NM_001143760.1
• Molecular Weight: 20,170 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 5A-1 isoform A
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 5A
• NCBI Official Symbol: EIF5A
• NCBI Official Synonym Symbols: EIF-5A; EIF5A1; eIF5AI
• NCBI Protein Information: eukaryotic translation initiation factor 5A-1; eIF-4D; eIF-5A-1; eIF-5A1; eukaryotic initiation factor 5A; rev-binding factor
• UniProt Protein Name: Eukaryotic translation initiation factor 5A-1
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF5A
• UniProt Synonym Gene Names: eIF-5A-1; eIF-5A1; eIF-5A
• UniProt Entry Name: IF5A1_HUMAN

Uniprot Description

EIF5A: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF- alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. Belongs to the eIF-5A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 17p13-p12

Cellular Component: endoplasmic reticulum membrane; membrane; cytoplasm; nuclear pore; cytosol; nucleus; annulate lamellae

Molecular Function: protein binding; RNA binding; ribosome binding; protein N-terminus binding; U6 snRNA binding; translation elongation factor activity

Biological Process: mRNA export from nucleus; cellular protein metabolic process; translational frameshifting; apoptosis; positive regulation of cell proliferation; nucleocytoplasmic transport; hypusine biosynthetic process from peptidyl-lysine; positive regulation of translational elongation; protein export from nucleus; post-translational protein modification; positive regulation of translational termination