Mouse anti-Human Estrogen Receptor alpha/ERalpha/ERS1 Monoclonal Antibody | anti-ESR1 antibody

Mouse anti Estrogen Receptor alpha/ERalpha/ERS1

Cat. Num. AAA570326

• Gene Names: ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
• Reactivity: Human
• Applications: Immunocytochemistry, Western Blot, Immunoprecipitation
• Synonyms: Estrogen Receptor alpha/ERalpha/ERS1; Monoclonal Antibody; Mouse anti Estrogen Receptor alpha/ERalpha/ERS1; anti-ESR1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: AER304
• Specificity: The antibody AER304 is directed against estrogen receptor alpha (ERalpha) and recognises amino acids residues 120 - 170, an epitope located in the B domain of ERalpha.
• Form/Format: Each vial contains 500 ul 0.2 mg/ml purified monoclonal antibody in phosphated buffered saline (PBS) containing 50% glycerol + 0.2% bovine serum albumin (BSA) + 0.02% sodium azide.
• Sequence Length: 595

• Applicable Applications for anti-ESR1 antibody: Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: The AER304 antibody is suitable for the detection of estrogen receptor alpha by western blotting, immunoprecipitation and immunocytochemistry. In western blotting, the antibody recognises a doublet of approximately 67 kDa in non-reducing conditions.; ; Optimal antibody dilutions for the different applications should be determined by titration.
• Source Note: AER304 is a mouse monoclonal IgG1 antibody derived by fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from a BALB/c mouse, immunized with estrogen receptor protein purified from calf uterus.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

Related Product Information for anti-ESR1 antibody

Estrogen receptor (ER) is a hormone binding molecule, which belongs to the nuclear hormone family of intracellular receptors. Estrogen receptors exist as alpha and beta forms encoded by separate genes ESR1 and ESR2. ERalpha and ERbeta share a high degree of sequence homology and are activated by the hormone 17beta-estradiol and related compounds, including phytoestrogens and xenoestrogens. Both ERalpha and ERbeta function as transcription factors which mediate many of the biological effects of estradiol at the level of gene regulation. Both receptors and are involved in the normal development and function of numerous tissues. Studies also suggest that ER's may be involved in a range of pathological conditions including cancers, neurodegenerative diseases and cardiovascular disease.

Product Categories/Family for anti-ESR1 antibody

Cancer; Apoptosis; Cell proliferation; Cell biology

References

1. Abbondanza C. et al. (1993) Characterization and epitope mapping of a new panel of monoclonal antibodies to estradiol receptor. Steroids. 58: 4-12.

NCBI and Uniprot Product Information

• NCBI GI #: 62821794
• NCBI GeneID: 2099
• NCBI Accession #: NP_000116.2
• NCBI GenBank Nucleotide #: NM_000125.3
• UniProt Accession #: P03372; Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7
• Molecular Weight: 35,547 Da
• NCBI Official Full Name: estrogen receptor isoform 1
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; ESTRR; NR3A1
• NCBI Protein Information: estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1
• UniProt Protein Name: Estrogen receptor
• Protein Family: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN

NCBI Description

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Uniprot Description

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; uterus development; signal transduction; response to estradiol stimulus; regulation of apoptosis; elevation of cytosolic calcium ion concentration; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of transcription factor activity; antral ovarian follicle growth; positive regulation of nitric-oxide synthase activity; androgen metabolic process; male gonad development; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); steroid hormone mediated signaling; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; gene expression

Disease: Breast Cancer; Migraine With Or Without Aura, Susceptibility To, 1; Myocardial Infarction, Susceptibility To; Estrogen Resistance