Mouse ESR2 Monoclonal Antibody | anti-ESR2 antibody

ESR2 Antibody

Cat. Num. AAA9200460

• Gene Names: ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Pig, Rat, Sheep)
• Applications: Western Blot, ELISA
• Synonyms: ESR2; Monoclonal Antibody; ESR2 Antibody; Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2; ESTRB; NR3A2; anti-ESR2 antibody

• Host: Mouse
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Pig, Rat, Sheep)
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 555CT18.5.5
• Specificity: This ESR2 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 369-397 amino acids from human ESR2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 369-397
• Sequence Length: 495

• Applicable Applications for anti-ESR2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:100~500
• Function: Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
• Cellular Location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407,ECO:0000269|PubMed:19126643,ECO:0000269|PubMed:20074560}
• Tissue Location: Isoform 1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta,liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform 2 is expressed in spleen, thymus, testis and ovaryand at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes,bone marrow, mammary gland and uterus. Isoform 4 is found in testis. Isoform 5 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform 6 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(ESR2 Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ESR2 antibody detected the ESR2 protein (arrow).)

Related Product Information for anti-ESR2 antibody

This gene encodes a member of the family of estrogen
receptors and superfamily of nuclear receptor transcription
factors. The gene product contains an N-terminal DNA binding domain
and C-terminal ligand binding domain and is localized to the
nucleus, cytoplasm, and mitochondria. Upon binding to
17beta-estradiol or related ligands, the encoded protein forms
homo- or hetero-dimers that interact with specific DNA sequences to
activate transcription. Some isoforms dominantly inhibit the
activity of other estrogen receptor family members. Several
alternatively spliced transcript variants of this gene have been
described, but the full-length nature of some of these variants has
not been fully characterized.

Product Categories/Family for anti-ESR2 antibody

Cancer; Metabolism; Neuroscience; Signal transduction

References

Fang, Y.J., et al. Tumour Biol. 31(6):651-658(2010)
Hayes, D.F., et al. Clin. Pharmacol. Ther. 88(5):626-629(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Wurster, M., et al. Oncol. Rep. 24(3):653-659(2010)
Paulus, J.K., et al. Lung Cancer (2010) In press :

NCBI and Uniprot Product Information

• NCBI GI #: 94538325
• NCBI GeneID: 2100
• NCBI Accession #: NP_001035365.1; NP_001201831.1; NP_001201832.1; NP_001258805.1; NP_001258806.1; NP_001428.1
• NCBI GenBank Nucleotide #: NM_001040275.1
• UniProt Accession #: Q92731; O60608; O60685; O60702; O60703; O75583; O75584; Q0MWT5; Q0MWT6; Q86Z31; A8K8K5; G3V5M5
• Molecular Weight: 59216
• NCBI Official Full Name: estrogen receptor beta isoform 2
• NCBI Official Synonym Full Names: estrogen receptor 2 (ER beta)
• NCBI Official Symbol: ESR2
• NCBI Official Synonym Symbols: Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA
• NCBI Protein Information: estrogen receptor beta
• UniProt Protein Name: Estrogen receptor beta
• Protein Family: Estrogen receptor
• UniProt Gene Name: ESR2
• UniProt Synonym Gene Names: ESTRB; NR3A2; ER-beta
• UniProt Entry Name: ESR2_HUMAN

NCBI Description

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

ER-beta: a nuclear hormone receptor and transcription factor. Binds and activated by estrogen. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Binds estrogens with an affinity similar to that of ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Eight alternatively-spliced isoforms have been described. Isoform beta-cx lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: nucleoplasm; mitochondrion; extracellular region; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor activity; receptor antagonist activity; estrogen receptor activity; enzyme binding; DNA binding; zinc ion binding; transcription coactivator activity; steroid hormone receptor activity; estrogen response element binding; transcription factor activity; steroid binding

Biological Process: transcription initiation from RNA polymerase II promoter; estrogen receptor signaling pathway; induction of apoptosis by hormones; neuron migration; vagina development; negative regulation of transcription from RNA polymerase II promoter; uterus development; signal transduction; cell-cell signaling; ovarian follicle development; regulation of transcription, DNA-dependent; gene expression; steroid hormone mediated signaling; positive regulation of transcription factor activity; negative regulation of cell growth; brain development; negative regulation of epithelial cell proliferation