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Mouse anti-E Coli Escherichia coli Monoclonal Antibody

Escherichia coli (E. coli) (Biotin)

Reactivity
E Coli
Applications
Immunoassay
Purity
Purified by Protein G Affinity Chromatography
Synonyms
Escherichia coli; Monoclonal Antibody; Escherichia coli (E. coli) (Biotin); anti-Escherichia coli antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
E Coli
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1.B.244
Specificity
Recognizes E. coli. No crossreactivity to: Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa or Serratia marcescens.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Biotin.
Applicable Applications for anti-Escherichia coli antibody
Immunoassay
Application Notes
Suitable for use in immunoassays to identify E. coli cells.
Applications are based on unconjugated antibody.
Immunogen
Pool of four E. coli serotypes common in human urinary tract infections: O18:B21:, O44:K74, O112:B11, O125:B15.
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Escherichia coli antibody
A strain of E. coli is a sub-group within the species that has unique characteristics that distinguish it from other E. coli strains. These differences are often detectable only on the molecular level; however, they may result in changes to the physiology or lifecycle of the bacterium. For example, a strain may gain pathogenic capacity, the ability to use a unique carbon source, the ability to inhabit a particular ecological niche or the ability to resist antimicrobial agents. Different strains of E. coli are often host-specific, making it possible to determine the source of fecal contamination in environmental samples.[6][7] For example, knowing which E. coli strains are present in a water sample allows to make assumptions about whether the contamination originated from a human, another mammal or a bird.
Product Categories/Family for anti-Escherichia coli antibody
References
1. "Escherichia coli O157:H7". CDC Division of Bacterial and Mycotic Diseases. Retrieved on 2007-01-25. 2. Vogt RL, Dippold L (2005). "Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June-July 2002". Public Health Rep 120 (2): 174-8. PMID 15842119. 3. Bentley R, Meganathan R (01 September 1982). "Biosynthesis of vitamin K (menaquinone) in bacteria". Microbiol. Rev. 46 (3): 241-80. PMID 6127606. PMC: 281544. 4. Hudault S, Guignot J, Servin AL (July 2001). "Escherichia coli strains colonising the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection". Gut 49 (1): 47-55. doi:10.1136/gut.49.1.47. PMID 11413110. 5. Reid G, Howard J, Gan BS (September 2001). "Can bacterial interference prevent infection?". Trends Microbiol. 9 (9): 424-8. doi:10.1016/S0966-842X(01)02132-1. PMID 11553454. 6. Feng P, Weagant S, Grant, M (2002-09-01). "Enumeration of Escherichia coli and the Coliform Bacteria". Bacteriological Analytical Manual (8th ed.). FDA/Center for Food Safety & Applied Nutrition. Retrieved on 2007-01-25. 7. Thompson, Andrea (2007-06-04). "E. coli Thrives in Beach Sands". Live Science. Retrieved on 2007-12-03. 8. "Escherichia". Taxonomy Browser. NCBI. Retrieved on 2007-11-30. 9. Kubitschek HE (01 January 1990). "Cell volume increase in Escherichia coli after shifts to richer media". J. Bacteriol. 172 (1): 94-101. PMID 2403552. PMC: 208405. 10. Madigan MT, Martinko JM (2006). Brock Biology of microorganisms (11th ed.). Pearson. ISBN 0-13-196893-9. 11. Fotadar U, Zaveloff P, Terracio L (2005). "Growth of Escherichia coli at elevated temperatures". J. Basic Microbiol. 45 (5): 403-4. doi:10.1002/jobm.200410542. PMID 16187264. 12. Ingledew WJ, Poole RK (1984). "The respiratory chains of Escherichia coli". Microbiol. Rev. 48 (3): 222-71. PMID 6387427. 13. Darnton NC, Turner L, Rojevsky S, Berg HC, On torque and tumbling in swimming Escherichia coli. J Bacteriol. 2007 Mar;18 Brussow H, Canchaya C, Hardt WD (September 2004). "Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion". Microbiol. Mol. Biol. Rev. 68 (3): 560-602. doi:10.1128/MMBR.68.3.560-602.2004. PMID 15353570. PMC: 515249.

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Product Notes

The Escherichia coli (Catalog #AAA6125111) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Escherichia coli (E. coli) (Biotin) reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's Escherichia coli can be used in a range of immunoassay formats including, but not limited to, Immunoassay. Suitable for use in immunoassays to identify E. coli cells. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Escherichia coli for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Escherichia coli, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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