Rabbit TNFSF4 Polyclonal Antibody | anti-TNFSF4 antibody

TNFSF4 Polyclonal Antibody

Cat. Num. AAA3007182

• Gene Names: TNFSF4; GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TNFSF4; Polyclonal Antibody; TNFSF4 Polyclonal Antibody; Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; CD252; TXGP1; anti-TNFSF4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TNFSF4 polyclonal antibody detects endogenous levels of TNFSF4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 183

• Applicable Applications for anti-TNFSF4 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human TNFSF4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TNFSF4 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysateLane2:H9C2 whole cell lysateLane3:SP20 whole cell lysate)

Related Product Information for anti-TNFSF4 antibody

The Ox40 ligand, Ox40L (also designated gp34), is a type II membrane protein, due to the absence of a signal peptide. Ox40L, a member of the tumor necrosis factor (TNF) superfamily, is a costimulatory molecule involved in dendritic cell: T cell interactions, T cell homing and B cell activation. Engagement of Ox40L with its receptor, Ox40, delivers a strong costimulatory signal to effector T cells. Ox40L is found preferentially on activated B cells and its receptor, Ox40, is a member of the tumor necrosis factor receptor (TNFR) family that is expressed on activated T cells. Ox40L plays a critical role in antigen-specific T cell responses in vivo and in both the priming and effector phases of T cell activation when expressed on antigen-presenting cells (APCs).

NCBI and Uniprot Product Information

• NCBI GI #: 4507603
• NCBI GeneID: 7292
• NCBI Accession #: NP_003317
• NCBI GenBank Nucleotide #: NP_003317.1
• UniProt Accession #: P23510
• Molecular Weight: ~ 15kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 4 isoform 1
• NCBI Official Synonym Full Names: TNF superfamily member 4
• NCBI Official Symbol: TNFSF4
• NCBI Official Synonym Symbols: GP34; CD252; OX4OL; TXGP1; CD134L; OX-40L; TNLG2B
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 4
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 4
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF4
• UniProt Synonym Gene Names: TXGP1; OX40L
• UniProt Entry Name: TNFL4_HUMAN

NCBI Description

This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

TNFSF4: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T-cell-APC interaction and the functional consequences of T-cell activation, thereby destabilizing peripheral tolerance. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Cell cycle regulation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: extracellular space; cell surface; integral to plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding; receptor binding

Biological Process: negative regulation of T-helper 1 cell differentiation; positive regulation of interleukin-12 production; negative regulation of cytokine secretion; positive regulation of T cell cytokine production; defense response to nematode; positive regulation of activated T cell proliferation; signal transduction; positive regulation of interleukin-10 production; positive regulation of interleukin-4 production; T cell proliferation; positive regulation of T-helper 2 cell differentiation; regulation of adaptive immune response; negative regulation of regulatory T cell differentiation; negative regulation of interferon-gamma production; positive regulation of cell proliferation; positive regulation of interleukin-13 production; positive regulation of memory T cell differentiation; cholesterol metabolic process; positive regulation of T-helper 2 type immune response; response to virus; negative regulation of transcription factor activity; positive regulation of immunoglobulin mediated immune response; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; positive regulation of CD4-positive, alpha beta T cell differentiation; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of B cell activation; regulation of inflammatory response; positive regulation of interleukin-2 production; positive regulation of alpha-beta T cell proliferation; immune response; negative regulation of transcription, DNA-dependent; acute inflammatory response; positive regulation of inflammatory response

Disease: Myocardial Infarction, Susceptibility To