ELISA (EIA)
(ELISA: A previous lot was use for the determination of Epo concentration in the range of 2-200ng/mL. Coat plate at 5ng/ml and 10ng/ml. Immunosorbent preparation)
Mouse anti-Human Erythropoietin Monoclonal Antibody | anti-EPO antibody
Erythropoietin (EPO, EP, Erythropoietin, MGC138142, MVCD2) (PE)
ELISA (EIA)
(ELISA: A previous lot was use for the determination of Epo concentration in the range of 2-200ng/mL. Coat plate at 5ng/ml and 10ng/ml. Immunosorbent preparation)
ELISA (EIA)
(ELISA: A previous lot was use for the determination of Epo concentration in the range of 2-200ng/mL. Coat plate at 5ng/ml and 10ng/ml. Immunosorbent preparation)
Testing Data
(Mouse kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with E3455-10L (1:500). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Arrow indicates glycosylated EPO (~34kD).)
Testing Data
(Mouse kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with E3455-10L (1:500). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Arrow indicates glycosylated EPO (~34kD).)
Western Blot (WB)
(Western Blot analysis: Mouse kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with E3455-10L (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates glycosylated EPO (~34kD).)
Western Blot (WB)
(Western Blot analysis: Mouse kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with E3455-10L (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates glycosylated EPO (~34kD).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]
Uniprot Description
EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7q22
Cellular Component: cell surface; extracellular region; extracellular space
Molecular Function: protein binding
Biological Process: erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of Ras protein signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein
Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2
Research Articles on EPO
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Product Notes
The EPO epo (Catalog #AAA6244583) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Erythropoietin (EPO, EP, Erythropoietin, MGC138142, MVCD2) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Erythropoietin can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the EPO epo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Erythropoietin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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