Rabbit anti-Human Fas Antibody | anti-Fas antibody

Fas Antibody

Cat. Num. AAA8503672

• Gene Names: FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: Fas; Fas Antibody; FAS; ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; TNFRSF6; anti-Fas antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 335

• Applicable Applications for anti-Fas antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human FAS
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of SW480cell lines, using FAS antibody.)

Related Product Information for anti-Fas antibody

Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1, 2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8-T lymphocytes (3, 4). FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-alpha, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDatrimeric soluble form (8, 9).

Product Categories/Family for anti-Fas antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Suda, T. et al. (1993) Cell 75, 1169-78. 2. Lee, H.O. and Ferguson, T.A. (2003) Cytokine Growth Factor Rev 14, 325-35. 3. Watanabe-Fukunaga, R. et al. (1992) Nature 356, 314-7. 4. Hahne, M. et al. (1995) IntImmunol 7, 1381-6. 5. Nagata, S. (1997) Cell 88, 355-65. 6. Green, D.R. and Ferguson, T.A. (2001) Nat Rev Mol Cell Biol 2, 917-24. 7. Walker, P.R. et al. (1997) J Immunol 158, 4521-4. 8. Kayagaki, N. et al. (1995) J Exp Med 182, 1777-83. 9. Tanaka, M. et al. (1995) EMBO J 14, 1129-35.

NCBI and Uniprot Product Information

• NCBI GI #: 4507583
• NCBI GeneID: 355
• NCBI Accession #: NP_000034.1
• NCBI GenBank Nucleotide #: NM_000043.5
• UniProt Accession #: P25445; Q14292; Q14293; Q14294; Q14295; Q16652; Q5T9P1; Q5T9P2; Q5T9P3; Q6SSE9; A9UJX4; B6VNV4
• Molecular Weight: 38kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 6 isoform 1
• NCBI Official Synonym Full Names: Fas cell surface death receptor
• NCBI Official Symbol: FAS
• NCBI Official Synonym Symbols: APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 6
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 6
• Protein Family: Fascin
• UniProt Gene Name: FAS
• UniProt Synonym Gene Names: APT1; FAS1; TNFRSF6

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]

Uniprot Description

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).