Western Blot (WB)
(All lanes : Anti-ERCC1 Antibody (C-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: T47D whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse anti-Human ERCC1 Monoclonal Antibody | anti-ERCC1 antibody
ERCC1 Antibody (C-term)
Western Blot (WB)
(All lanes : Anti-ERCC1 Antibody (C-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: T47D whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-ERCC1 Antibody (C-term) at 1:2000 dilutionLane 1: A549 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: T47D whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 33 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of lysates from A549, T47D cell line (from left to right), using ERCC1 Antibody (C-term). MBS9200614 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from A549, T47D cell line (from left to right), using ERCC1 Antibody (C-term). MBS9200614 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Western Blot (WB)
(ERCC1 Antibody (C-term) western blot analysis in Hela,A549 cell line lysates (35ug/lane).This demonstrates the ERCC1 antibody detected the ERCC1 protein (arrow).)
Western Blot (WB)
(ERCC1 Antibody (C-term) western blot analysis in Hela,A549 cell line lysates (35ug/lane).This demonstrates the ERCC1 antibody detected the ERCC1 protein (arrow).)
Hoeijmakers J.H.J., et al. Cold Spring Harb. Symp. Quant. Biol. 51:91-101(1986).
Yu J.J., et al. Mutat. Res. 382:13-20(1997).
Hisatomi H., et al. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI Description
The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]
Uniprot Description
ERCC1: a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA excision repair. Belongs to the ERCC1/RAD10/SWI10 family. Heterodimer composed of ERCC1 and XPF/ERRC4. Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4, a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. Low levels of this protein are associated with increased sensitivity to cisplatin. The overall survival of non-small cell lung cancer patients with single-nucleotide polymorphisms at codon 118 receiving platinum-based chemotherapy was significantly improved.
Protein type: DNA repair, damage
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: nucleoplasm; transcription factor TFIID complex; nucleotide-excision repair complex; nuclear chromosome, telomeric region; cytoplasm
Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; structure-specific DNA binding; single-stranded DNA specific endodeoxyribonuclease activity; TATA-binding protein binding; damaged DNA binding; single-stranded DNA binding
Biological Process: oogenesis; chromosome organization and biogenesis; DNA recombination; negative regulation of telomere maintenance; UV protection; post-embryonic hemopoiesis; double-strand break repair; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; nucleotide-excision repair, DNA incision, 5'-to lesion; response to nutrient; response to X-ray; mitotic recombination; multicellular organism growth; male gonad development; pyrimidine dimer repair via nucleotide-excision repair; isotype switching; multicellular organismal aging; replicative cell aging; DNA repair; DNA catabolic process, endonucleolytic; syncytium formation; response to sucrose stimulus; nucleotide-excision repair, DNA incision, 3'-to lesion; cell proliferation; nucleotide-excision repair; embryonic organ development; spermatogenesis; response to oxidative stress
Disease: Cerebrooculofacioskeletal Syndrome 4
Research Articles on ERCC1
Similar Products
Product Notes
The ERCC1 ercc1 (Catalog #AAA9200614) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 268-297. The ERCC1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERCC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the ERCC1 ercc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERCC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
