Mouse anti-Human ErbB-2/HER-2 Monoclonal Antibody | anti-ErbB-2/HER-2 antibody

Anti-ErbB-2/HER-2 Mouse mAb

Cat. Num. AAA477214

• Gene Names: ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• Reactivity: Human
• Applications: Western Blot
• Purity: Fractional precipitation
• Synonyms: ErbB-2/HER-2; Monoclonal Antibody; Anti-ErbB-2/HER-2 Mouse mAb; anti-ErbB-2/HER-2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Purity/Purification: Fractional precipitation
• Form/Format: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• Sequence Length: 1225

• Applicable Applications for anti-ErbB-2/HER-2 antibody: Western Blot (WB)
• Application Notes: WB: 1:2,000-4,000; IHC: 1:200
• Immunogen: Synthetic Peptide
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of 1) Hela, 2)Mouse Brain with ErbB-2/HER-2 mAb diluted at 1:4, 000.)

Immunohistochemistry (IHC)

(IHC staining of human breast cancer tissue with ErbB-2/HER-2 mouse mAb(11H9) diluted at 1:200.)

NCBI and Uniprot Product Information

• NCBI GI #: 54792098
• NCBI GeneID: 2064
• NCBI Accession #: NP_001005862.1
• NCBI GenBank Nucleotide #: NM_001005862.2
• UniProt Accession #: P04626; Q14256; Q6LDV1; Q9UMK4; B2RZG3; B4DHN3; X5D2V5
• Molecular Weight: 138
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-2 isoform b
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 2
• NCBI Official Symbol: ERBB2
• NCBI Official Synonym Symbols: NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-2
• UniProt Gene Name: ERBB2
• UniProt Synonym Gene Names: HER2; MLN19; NEU; NGL; MLN 19

NCBI Description

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.