Testing Data
(Fig.1: Mass spectroscopic analysis of EpoR immuno-precipitated from UT7 cells by GM-1201 or GM-1202 that were further analysed on denaturing SDS-PAGE gels (identified in Western blot with GM-1201). Segments of the gel were cut out for analysis by mass spectroscopy. For detailed information see Reference 5.)
Epo-R Monoclonal Antibody | anti-EPOR antibody
Rat anti-human Epo-R monoclonal antibody
ELISA: 1:200 - 1:400
C-ELISA only on permeabilised cells: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Long term:-20°C
(Avoid repeated freezing and thawing)
Testing Data
(Fig.1: Mass spectroscopic analysis of EpoR immuno-precipitated from UT7 cells by GM-1201 or GM-1202 that were further analysed on denaturing SDS-PAGE gels (identified in Western blot with GM-1201). Segments of the gel were cut out for analysis by mass spectroscopy. For detailed information see Reference 5.)
Testing Data
(Fig.1: Mass spectroscopic analysis of EpoR immuno-precipitated from UT7 cells by GM-1201 or GM-1202 that were further analysed on denaturing SDS-PAGE gels (identified in Western blot with GM-1201). Segments of the gel were cut out for analysis by mass spectroscopy. For detailed information see Reference 5.)
Testing Data
(Fig.2: CGE analysis of purified GM1201 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified GM1201 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Testing Data
(Fig.2: CGE analysis of purified GM1201 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 mug of purified GM1201 antibody. Proteins were separated by CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Erythropoietin receptor (Epo-R) belongs to the cytokine receptor family and is a 507 amino acid type I transmembrane protein. EpoR pre-exists as dimers which changes the homodimerized state after binding of its 34 kDa ligand erythropoietin (Epo) (1,2). Erythropoietin is the primary regulator of erythropoiesis, and promotes the survival, proliferation, and differentiation of erythroid progenitor cells. Both, Epo and Epo-R are essential for the production of red blood cells due to Epo exerts its function through the Epo receptor (3). The Epo-R is also expressed in many organs outside the bone marrow, suggesting that Epo is a pleiotropic anti-apoptotic factor. Signaling pathways have been shown to influence numerous cellular functions in normal and tumor cells, including proliferation, apoptosis, and drug resistance (4). Development and specificity testing of the GM-1201 antibody are described in detail elsewhere (5).
2. Livnah O, Stura EA, Middleton SA, Johnson DL, Jolliffe LK, Wilson IA (1999). Crystallographic evidence for preformed dimers of erythropoietin receptor before ligand activation. Science 283 (5404): 987-90
3. Wilson IA, Jolliffe LK (1999). The structure, organization, activation and plasticity of the erythropoietin receptor. Curr Opin Struct Biol.;9(6): 696-704
4. Hedley BD, Allan AL, Xenocostas A (2011). The role of erythropoietin and erythropoiesisstimulating agents in tumor progression. Clin Cancer Res. 15; 17(20): 6373-80
5. Maxwell P et al. (2015). Novel antibodies directed against the human erythropoietin receptor: creating a basis for clinical implementation. Br J Haematol; 168(3):429-42. doi: 10.1111/bjh.13133. Epub 2014 Oct 4.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Uniprot Description
EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 19p13.3-p13.2
Cellular Component: cytosol; external side of plasma membrane; extracellular region; integral to plasma membrane
Molecular Function: erythropoietin receptor activity; identical protein binding; protein binding
Biological Process: brain development; decidualization; elevation of cytosolic calcium ion concentration; heart development; negative regulation of neuron apoptosis; positive regulation of cell proliferation; signal transduction
Disease: Erythrocytosis, Familial, 1
Research Articles on EPOR
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Product Notes
The EPOR epor (Catalog #AAA468055) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Epo-R can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), C-ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP). Flow Cytometry only on permeabilised cells: 1.2 ug/10^6 cells ELISA: 1:200 - 1:400 C-ELISA only on permeabilised cells: 1:200 - 1:400For each application a titration should be performed to determine the optimal concentration. Researchers should empirically determine the suitability of the EPOR epor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Epo-R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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