Testing Data
(Detection of mouse EphB2 |Lysates were prepared from mouse brain |extract in non-reducing sample buffer, |resolved by SDS-PAGE (30ug total |protein/lane), and transferred to an |Immobilon-P membrane. The blot was |developed with 2ug/ml and |chemiluminescent detection substrate.)
Rat anti-Mouse EphB2 Monoclonal Antibody | anti-EPHB2 antibody
EphB2 (EPH receptor B2, CAPB, DRT, Ephrin type-B receptor 2, EPHT3, EPTH3, ERK, Hek5, HEK5, MGC87492, PCBC, Receptor protein-tyrosine kinase HEK5, Renal carcinoma antigen NY-REN-47, Tyro5, TYRO5, Tyrosine-protein kinase receptor EPH-3, Tyrosine-protein ki
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 2ug/ml
Testing Data
(Detection of mouse EphB2 |Lysates were prepared from mouse brain |extract in non-reducing sample buffer, |resolved by SDS-PAGE (30ug total |protein/lane), and transferred to an |Immobilon-P membrane. The blot was |developed with 2ug/ml and |chemiluminescent detection substrate.)
Testing Data
(Detection of mouse EphB2 |Lysates were prepared from mouse brain |extract in non-reducing sample buffer, |resolved by SDS-PAGE (30ug total |protein/lane), and transferred to an |Immobilon-P membrane. The blot was |developed with 2ug/ml and |chemiluminescent detection substrate.)
NCBI and Uniprot Product Information
NCBI Description
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq]
Uniprot Description
EphB2: a receptor tyrosine kinase of the Eph family. A receptor for ephrin-B family members. Activated EphB2 recruits RasGAP, down-regulating the Ras-Erk signaling axis and neurite retraction. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB2 interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. Point mutations seen in prostate cancer. Overexpressed and required for migration of glioblastoma. Overexpressed and correlated with poor survival in breast cancer. Overexpression and loss of heterozygosity seen in colorectal cancers. Target for immunoconjugate drug therapy .Three splice-variant isoforms have been described.
Protein type: Protein kinase, tyrosine (receptor); Kinase, protein; Tumor suppressor; EC 2.7.10.1; Protein kinase, TK; Membrane protein, integral; TK group; Eph family
Chromosomal Location of Human Ortholog: 1p36.1-p35
Cellular Component: cell soma; axon; integral to plasma membrane; dendrite; extracellular region; plasma membrane; cytosol
Molecular Function: protein binding; transmembrane-ephrin receptor activity; protein-tyrosine kinase activity; axon guidance receptor activity; ATP binding; receptor binding
Biological Process: axon guidance; nervous system development; inner ear morphogenesis; peptidyl-tyrosine phosphorylation; axonal fasciculation; central nervous system projection neuron axonogenesis; learning; palate development; optic nerve morphogenesis; positive regulation of long-term neuronal synaptic plasticity; corpus callosum development; positive regulation of synaptogenesis; regulation of body fluid levels; camera-type eye morphogenesis; ephrin receptor signaling pathway; negative regulation of axonogenesis; angiogenesis; phosphorylation; urogenital system development; retinal ganglion cell axon guidance
Disease: Prostate Cancer/brain Cancer Susceptibility
Research Articles on EPHB2
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Product Notes
The EPHB2 ephb2 (Catalog #AAA606713) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The EphB2 (EPH receptor B2, CAPB, DRT, Ephrin type-B receptor 2, EPHT3, EPTH3, ERK, Hek5, HEK5, MGC87492, PCBC, Receptor protein-tyrosine kinase HEK5, Renal carcinoma antigen NY-REN-47, Tyro5, TYRO5, Tyrosine-protein kinase receptor EPH-3, Tyrosine-protein ki reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EphB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 2ug/ml. Researchers should empirically determine the suitability of the EPHB2 ephb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EphB2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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