Rat anti-Human EPCR Monoclonal Antibody | anti-EPCR antibody

EPCR, Human, mAb RCR-252

Cat. Num. AAA584008

• Gene Names: PROCR; CCCA; EPCR; CCD41
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Synonyms: EPCR; Monoclonal Antibody; Human; mAb RCR-252; Activated protein C receptor; CD201; Endothelial cell protein C receptor; anti-EPCR antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rat IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 238

• Applicable Applications for anti-EPCR antibody: Flow Cytomery(FC/FACS), FS, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Application Notes: FC: Antibody RCR-252 stains the extracellular domain of human EPCR. As positive control RE-1 cells were used and as negative control N1 cells. (Ref.1); Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro a dilution of 1:5 is advised as start concentration.
• Immunogen: Human EPCR-positive RE-1 cells
• Positive Control: -RE-1 (rat 3Y1 fibroblasts stably transfected with human EPCR.
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-EPCR antibody

Product Description: The monoclonal antibody RCR-252 recognizes human endothelial protein C receptor (EPCR), a highly glycosylated type I transmembrane protein of 221-amino-acids. These amino acids comprise an extracellular domain, a 25-aa transmembrane domain, and a short (3 aa) intracytoplasmic sequence coding for an ~46 kDa protein. Deglycosylation will reduce the protein mass to 25 kDa. EPCR is expressed strongly on the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.
EPCR is the receptor for protein C, a key player in the anticoagulation pathway. The protein C anticoagulant pathway serves as a major system for controling thrombosis, limiting inflammatory responses, and potentially decreasing endothelial cell apoptosis in response to inflammatory cytokines and ischemia. The essential components of the pathway include thrombin, thrombomodulin, the endothelial cell protein C receptor (EPCR), protein C and protein S. The pathway is initiated when thrombin binds to thrombomodulin on the surface of endothelium. EPCR augments protein C activation by binding protein C and presenting it to the thrombin-thrombomodulin activation complex. Activated protein C (aPC) retains its ability to bind EPCR, and this complex appears to be involved in some of the cellular signaling mechanisms that down-regulate inflammatory cytokine formation (TNF, IL-6). EPCR is shed from the vasculature by inflammatory mediators and thrombin. EPCR binds to activated neutrophils in a process that involves proteinase 3 and Mac-1. Furthermore, EPCR can undergo translocation from the plasma membrane to the nucleus.
EPCR can be cleaved to release a soluble form (sEPCR) in the circulation. This sEPCR is detected as a single species of 43 kDa, resulting from shedding of membrane EPCR by the action of a metalloprotease,- which is stimulated by thrombin and by some inflammatory mediators. Soluble EPCR binds PC and aPC with similar affinity, but its binding to aPC inhibits the anticoagulant activity of aPC by blocking its binding to phospholipids and by abrogating its ability to inactivate factor Va. sEPCR- can be detected in plasma. In normal persons, sEPCR is present in levels of 83.6 +/- 17.2 ng/ml. Elevated levels of sEPCR are positively correlated to a higher risk for thrombosis. Furthermore, a haplotype (A3 allele) has been linked to elevated levels of sEPCR (264 +/-174 ng/ml).

References

1. Ye, X et al; The endothelial cell protein C receptor (EPCR) functions as a primary receptor for protein C activation on endothelial cells in arteries, veins, and capillaries. Biochem Biophys Res Commun 1999, 259: 671 2. Dömötör, E et al; Activated protein C alters cytosolic calcium flux in human brain endothelium via binding to endothelial protein C receptor and activation of protease activated receptor-1. Blood 2003, 101: 4797
3. Sturn, D et al; Expression and function of the endothelial protein C receptor in human neutrophils. Blood 2003, 102: 1499
4. Finigan, J et al; Activated protein C mediates novel lung endothelial barriere enhancement. J Biol Chem 2005, 280: 17286
5. Bae J et al; Receptors of the protein C activation and activated protein C signaling pathways are colocalized in lipid rafts of endothelial cells. PNAS 2006, 104: 2867

NCBI and Uniprot Product Information

• NCBI GI #: 34335272
• NCBI GeneID: 10544
• NCBI Accession #: NP_006395.2
• NCBI GenBank Nucleotide #: NM_006404.4
• Molecular Weight: 26,671 Da
• NCBI Official Full Name: endothelial protein C receptor
• NCBI Official Synonym Full Names: protein C receptor, endothelial
• NCBI Official Symbol: PROCR
• NCBI Official Synonym Symbols: CCCA; EPCR; CCD41
• NCBI Protein Information: endothelial protein C receptor; APC receptor; centrocyclin; CD201 antigen; activated protein C receptor; cell cycle, centrosome-associated protein
• UniProt Protein Name: Endothelial protein C receptor
• UniProt Gene Name: PROCR
• UniProt Synonym Gene Names: EPCR; APC receptor
• UniProt Entry Name: EPCR_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer. [provided by RefSeq, Jul 2013]

Uniprot Description

EPCR: a single-pass type I membrane protein. Binds activated protein C (APC), a serine protease that regulates thrombin (IIa) production. Plays a pivotal role in preventing blood coagulation through binding of activated protein C. Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. The binding of APC to EPCR and PAR-1 increases invasion and chemotaxis.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: centrosome; cell surface; focal adhesion; integral to plasma membrane; extracellular region; plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: antigen processing and presentation; immune response; blood coagulation; negative regulation of coagulation; leukocyte migration