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Immunohistochemistry (IHC)
(Immunochemical staining of mouse EpCAM in mouse uterus with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Mouse EpCAM Monoclonal Antibody | anti-EpCAM antibody
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal
Gene Names
Epcam; EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; EpCAM1; Tacsd1; GA733-2; Tacstd1
Reactivity
Mouse
Applications
Western Blot, ELISA, ELISA, Immunohistochemistry
Purity
Protein A
Synonyms
EpCAM; Monoclonal Antibody; Recombinant Anti-EpCAM Antibody; Rabbit Monoclonal; EpCAM/TROP-1/TACSTD1 Antibody; Rabbit MAb; epithelial cell adhesion molecule; Anti-CD326 Antibody; Anti-EGP Antibody; Anti-EGP-2 Antibody; Anti-Egp314 Antibody; Anti-Ep-CAM Antibody; Anti-EpCAM1 Antibody; Anti-GA733-2 Antibody; Anti-gp40 Antibody; Anti-Ly74 Antibody; Anti-Tacsd1 Antibody; Anti-Tacstd1 Antibody; Anti-TROP1 Antibody; anti-EpCAM antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
002
Specificity
Mouse EpCAM/TROP-1/TACSTD1.
No cross-reactivity in ELISA with Human EPCAM
No cross-reactivity in ELISA with Human EPCAM
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-EpCAM antibody
Western Blot (WB), ELISA (EIA), ELISA (EIA) (Det), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:1000
ELISA: 1:25,000-1:50,000
IHC-P: 1:500-1:2000
ELISA (Det): 1:5000-1:50,000
ELISA (Det): This antibody will detect Mouse EpCAM/TROP-1/TACSTD1 in ELISA pair set.
In a sandwich ELISA, it can be used as detection antibody when paired with.
ELISA: 1:25,000-1:50,000
IHC-P: 1:500-1:2000
ELISA (Det): 1:5000-1:50,000
ELISA (Det): This antibody will detect Mouse EpCAM/TROP-1/TACSTD1 in ELISA pair set.
In a sandwich ELISA, it can be used as detection antibody when paired with.
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse EpCAM/TROP-1/TACSTD1 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse EpCAM/TROP-1/TACSTD1 (rM EpCAM/TROP-1/TACSTD1).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse EpCAM in mouse uterus with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse EpCAM in mouse uterus with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse EpCAM in mouse stomach with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse EpCAM in mouse stomach with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse EpCAM in mouse intestine with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse EpCAM in mouse intestine with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Western Blot (WB)
(Anti-EPCAM rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:38 kDa)
Western Blot (WB)
(Anti-EPCAM rabbit monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDaObserved band size:38 kDa)
Related Product Information for anti-EpCAM antibody
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
References
Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,019 Da
NCBI Official Full Name
epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
Epcam
NCBI Official Synonym Symbols
EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; EpCAM1; Tacsd1; GA733-2; Tacstd1
NCBI Protein Information
epithelial cell adhesion molecule; mEGP314; protein 289A; Trop-1 protein; lymphocyte antigen 74; epithelial glycoprotein 314; panepithelial glycoprotein 314; tumor-associated calcium signal transducer 1
UniProt Protein Name
Epithelial cell adhesion molecule
Protein Family
UniProt Gene Name
Epcam
UniProt Synonym Gene Names
Tacstd1; Ep-CAM; EGP314; mEGP314
UniProt Entry Name
EPCAM_MOUSE
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Product Notes
The EpCAM epcam (Catalog #AAA8111814) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EpCAM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), ELISA (EIA) (Det), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:1000 ELISA: 1:25,000-1:50,000 IHC-P: 1:500-1:2000 ELISA (Det): 1:5000-1:50,000 ELISA (Det): This antibody will detect Mouse EpCAM/TROP-1/TACSTD1 in ELISA pair set. In a sandwich ELISA, it can be used as detection antibody when paired with. Researchers should empirically determine the suitability of the EpCAM epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EpCAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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