Western Blot (WB)
(Western Blot analysis of Rat Colon lysate probed with Ep-CAM Mouse Monoclonal Antibody (GZ-1). )
Mouse Ep-CAM/CD326 Monoclonal Antibody | anti-Ep-CAM/CD326 antibody
Ep-CAM/CD326 (Rat) Mouse Monoclonal Antibody [Clone GZ-1]
Gene Names
Epcam; Egp314; Tacstd1
Reactivity
RatDoes not react with Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunoprecipitation
Synonyms
Ep-CAM/CD326; Monoclonal Antibody; Ep-CAM/CD326 (Rat) Mouse Monoclonal Antibody [Clone GZ-1]; Ep-CAM/CD326 (Rat); Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1; ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; M17S1 (Membrane Component; Chromosome 17; Surface Marker-1); REG-1; Reggie-1; Reggie-2; anti-Ep-CAM/CD326 antibody
Host
Mouse
Reactivity
Rat
Does not react with Human
Does not react with Human
Clonality
Monoclonal
Isotype
Mouse/IgG1, kappa
Clone Number
GZ-1
Form/Format
Bioreactor Concentrate with 0. 05% Azide.
Applicable Applications for anti-Ep-CAM/CD326 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
FC/FACS: 5-10ul/million cells
IF: 1:50-1:100)
WB: 1:100-1:200)
IP: 5-10ul/500ug protein lysate)
IF: 1:50-1:100)
WB: 1:100-1:200)
IP: 5-10ul/500ug protein lysate)
Immunogen
Rat small intestine cells
Cellular Localization
Cell surface
Positive Control
Rat small intestine or colon. Rat colon lysate.
Preparation and Storage
Store at 2 to 8 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Western Blot (WB)
(Western Blot analysis of Rat Colon lysate probed with Ep-CAM Mouse Monoclonal Antibody (GZ-1). )
Western Blot (WB)
(Western Blot analysis of Rat Colon lysate probed with Ep-CAM Mouse Monoclonal Antibody (GZ-1). )
Related Product Information for anti-Ep-CAM/CD326 antibody
Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on basolateral cell surface in most simple epithelia and a vast majority of carcinomas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,207 Da
NCBI Official Full Name
epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
Epcam
NCBI Official Synonym Symbols
Egp314; Tacstd1
NCBI Protein Information
epithelial cell adhesion molecule; ep-CAM; epithelial glycoprotein 314; tumor-associated calcium signal transducer 1
UniProt Protein Name
Epithelial cell adhesion molecule
UniProt Gene Name
Epcam
UniProt Synonym Gene Names
Tacstd1; Ep-CAM; EGP314
UniProt Entry Name
EPCAM_RAT
NCBI Description
involved in metastasis formation and tumor progression [RGD, Feb 2006]
Uniprot Description
Function: May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E
By similarity.
Subunit structure: Monomer
By similarity. Interacts with phosphorylated CLDN7. Ref.4
Subcellular location: Lateral cell membrane; Single-pass type I membrane protein. Cell junction › tight junction. Note: Co-localizes with CLDN7 at the lateral cell membrane and tight junction. Ref.4
Post-translational modification: Glycosylation at Asn-198 is crucial for protein stability
By similarity.
Sequence similarities: Belongs to the EPCAM family.Contains 1 thyroglobulin type-1 domain.
Research Articles on Ep-CAM/CD326
Similar Products
Product Notes
The Ep-CAM/CD326 epcam (Catalog #AAA4382434) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ep-CAM/CD326 (Rat) Mouse Monoclonal Antibody [Clone GZ-1] reacts with Rat Does not react with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ep-CAM/CD326 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP). FC/FACS: 5-10ul/million cells IF: 1:50-1:100) WB: 1:100-1:200) IP: 5-10ul/500ug protein lysate). Researchers should empirically determine the suitability of the Ep-CAM/CD326 epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ep-CAM/CD326, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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