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Western Blot (WB) (Western Blot detection against Immunogen (36.52kD).)

Mouse anti-Human ENPP3 Monoclonal Antibody | anti-ENPP3 antibody

ENPP3 (PDNP3, Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 3, Phosphodiesterase I beta, Phosphodiesterase I/Nucleotide Pyrophosphatase 3, CD203c, Alkaline Phosphodiesterase I, Nucleotide Pyrophosphatase)

Gene Names
ENPP3; B10; NPP3; PDNP3; CD203c; PD-IBETA
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
ENPP3; Monoclonal Antibody; ENPP3 (PDNP3; Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 3; Phosphodiesterase I beta; Phosphodiesterase I/Nucleotide Pyrophosphatase 3; CD203c; Alkaline Phosphodiesterase I; Nucleotide Pyrophosphatase); Anti -ENPP3 (PDNP3; anti-ENPP3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1G11
Specificity
Recognizes human ENPP3.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
ATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASN
Applicable Applications for anti-ENPP3 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Immunogen
Partial recombinant corresponding to aa602-700 from human ENPP3 (NP_005012) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.52kD).)

Western Blot (WB) (Western Blot detection against Immunogen (36.52kD).)

Testing Data

(Detection limit for recombinant GST tagged ENPP3 is 0.1ng/ml as a capture antibody)

Testing Data (Detection limit for recombinant GST tagged ENPP3 is 0.1ng/ml as a capture antibody)
Related Product Information for anti-ENPP3 antibody
The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. [provided by RefSeq].
Product Categories/Family for anti-ENPP3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
100,124 Da
NCBI Official Full Name
ENPP3 protein
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 3
NCBI Official Symbol
ENPP3
NCBI Official Synonym Symbols
B10; NPP3; PDNP3; CD203c; PD-IBETA
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 3; E-NPP 3; gp130RB13-6; phosphodiesterase I beta; phosphodiesterase-I beta; phosphodiesterase I/nucleotide pyrophosphatase 3; dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3)
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3
UniProt Gene Name
ENPP3
UniProt Synonym Gene Names
PDNP3; E-NPP 3; PD-Ibeta; NPPase
UniProt Entry Name
ENPP3_HUMAN

NCBI Description

The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD

By similarity.

Catalytic activity: Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.A dinucleotide + H2O = 2 mononucleotides.

Cofactor: Binds 2 divalent metal cations per subunit

Probable.

Enzyme regulation: At low concentrations of ATP, a phosphorylated active site intermediate is formed which inhibits further ATP hydrolysis.

Subcellular location: Membrane; Single-pass type II membrane protein

Potential. Secreted. Note: Located to the apical surface in intestinal and kidney epithelial cells. Located to the cell surface of basophils, and to the apical plasma membrane of bile duct cells. Secreted in serum, and in lumen of epithelial cells. Ref.5 Ref.6

Tissue specificity: Expressed in bile ducts, prostate, uterus and colon. Exclusively expressed on basophils, mast cells and their progenitors. Ref.1 Ref.6

Induction: Up-regulated by stimulation by allergen or by cross-linking with IgE. The IgE-mediated activation is enhanced by tetradecanoyl phorbol acetate (TPA), a stimulator of the PKC pathway, and inhibited by the P13 kinase inhibitors, LY294002 and wortmannin. Up-regulated in invasive bile duct cancers. Ref.7

Post-translational modification: N-glycosylation is necessary for correct trafficking to the apical surface, but is not the apical targeting signal

By similarity.It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.

Sequence similarities: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.Contains 2 SMB (somatomedin-B) domains.

Sequence caution: The sequence AAD05192.1 differs from that shown. Reason: Erroneous gene model prediction.

Research Articles on ENPP3

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Product Notes

The ENPP3 enpp3 (Catalog #AAA648788) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ENPP3 (PDNP3, Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 3, Phosphodiesterase I beta, Phosphodiesterase I/Nucleotide Pyrophosphatase 3, CD203c, Alkaline Phosphodiesterase I, Nucleotide Pyrophosphatase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ENPP3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the ENPP3 enpp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ATVKVNLPFG RPRVLQKNVD HCLLYHREYV SGFGKAMRMP MWSSYTVPQL GDTSPLPPTV PDCLRADVRV PPSESQKCSF YLADKNITHG FLYPPASN. It is sometimes possible for the material contained within the vial of "ENPP3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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