Western Blot (WB)
(Western Blot Lane1: Human Hepg2 Cells Lane2: Human Hela Cells Primary Ab: 2ug/mL Rabbit Anti-Human NSE Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab )
Mouse anti-Human Enolase, Neuron Specific (NSE) Monoclonal Antibody | anti-NSE antibody
Monoclonal Antibody to Enolase, Neuron Specific (NSE)
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot Lane1: Human Hepg2 Cells Lane2: Human Hela Cells Primary Ab: 2ug/mL Rabbit Anti-Human NSE Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab )
Western Blot (WB)
(Western Blot Lane1: Human Hepg2 Cells Lane2: Human Hela Cells Primary Ab: 2ug/mL Rabbit Anti-Human NSE Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P Samples:Human Lung Cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P Samples:Human Lung Cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples:Human Pancreas Cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples:Human Pancreas Cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P Samples:Human Breast Cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P Samples:Human Breast Cancer Tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Uniprot Description
ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: cytosol; extracellular exosome; extracellular space; membrane; myelin sheath; perikaryon; phosphopyruvate hydratase complex; photoreceptor inner segment; plasma membrane
Molecular Function: magnesium ion binding; phosphopyruvate hydratase activity; protein binding
Biological Process: gluconeogenesis; glycolytic process
Research Articles on NSE
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Product Notes
The NSE eno2 (Catalog #AAA2085852) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Enolase, Neuron Specific (NSE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Enolase, Neuron Specific (NSE) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/ml Immunocytochemistry in formalin fixed cells: 5-30ug/ml Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml Immunohistochemistry in paraffin section: 5-30ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Researchers should empirically determine the suitability of the NSE eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Enolase, Neuron Specific (NSE), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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