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Western Blot (WB) (Western blot analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa))

Mouse anti-Human, Mouse ENO2 Monoclonal Antibody | anti-ENO2 antibody

Anti-ENO2 Mouse mAb

Gene Names
ENO2; NSE; HEL-S-279
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
ENO2; Monoclonal Antibody; Anti-ENO2 Mouse mAb; NSE; anti-ENO2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5D3
Form/Format
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Sequence Length
434
Applicable Applications for anti-ENO2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Immunogen
Purified recombinant fragment of human ENO2 (AA: 251-433) expressed in E Coli.

Western Blot (WB)

(Western blot analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa))

Western Blot (WB) (Western blot analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa))

Western Blot (WB)

(Western blot analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.)

Western Blot (WB) (Western blot analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ENO2 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ENO2 mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ENO2 mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ENO2 mouse mAb with DAB staining.)
Related Product Information for anti-ENO2 antibody
Entrez Summary: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
47.3
NCBI Official Full Name
ENO2
NCBI Official Synonym Full Names
enolase 2
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
NCBI Protein Information
gamma-enolase
UniProt Protein Name
Gamma-enolase
Protein Family
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE

NCBI Description

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

Uniprot Description

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytosol; extracellular space

Molecular Function: phosphopyruvate hydratase activity; protein binding

Biological Process: gluconeogenesis

Research Articles on ENO2

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Product Notes

The ENO2 eno2 (Catalog #AAA476331) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ENO2 Mouse mAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ENO2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the ENO2 eno2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENO2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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