Mouse anti-Human, Rat Endothelial Monocyte Activating Polypeptide II Monoclonal Antibody | anti-AIMP1 antibody

Endothelial Monocyte Activating Polypeptide II (EMAP II)

Cat. Num. AAA603358

• Gene Names: AIMP1; p43; HLD3; EMAP2; SCYE1; EMAPII
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified
• Synonyms: Endothelial Monocyte Activating Polypeptide II; Monoclonal Antibody; Endothelial Monocyte Activating Polypeptide II (EMAP II); Anti -Endothelial Monocyte Activating Polypeptide II (EMAP II); anti-AIMP1 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 6A542
• Specificity: Recognizes human endothelial-monocyte-activating polypeptide II (EMAP II) at ~20kD. Species Crossreactivity: Rat
• Purity/Purification: Affinity Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.

• Applicable Applications for anti-AIMP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, ELISA and Immunohistochemistry.; Dilution: Immunohistology (Paraffin): 1:10; Flow Cytometry: 1:10; Western Blot: 1:10; ELISA: Detection
• Immunogen: Human Endothelial Monocyte Activating Polypeptide II (EMAP II).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-AIMP1 antibody

EMAP II is an antiangiogenic and proinflammatory cytokine (expressed by microglia/macrophages). EMAP II appears to be a profound modulator of inflammatory reactions of the innate immune system and might participate in a variety of pathogenic immune-processes in the mammalian brain. EMAP II is a potential mediator of inflammatory responses in autoimmunity and a considered target to stage reactions of monocytic lineage cells in inflammatory reactions. In normal tissues EMAP II is expressed in endocrine organs, in cells of neuroendocrine origin, but also in tissues with high cell turnover. Under pathophysiological conditions EMAP II has several functions. It triggers the recruitment of macrophages by elevating cytosolic-free Ca2+ concentrations, it stimulates leukocyte chemotaxis, it induces the expression of TNF-alpha and tissue factor (TF) by monocytes and myeloperoxidase (MPO) by polymorphonuclear cells (PNMs). Furthermore it stimulates the release of von Willebrand-factor, P- and E-selectin by endothelial cells and EMAP II induces endothelial programmed cell death in vitro.

Product Categories/Family for anti-AIMP1 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 498910
• NCBI GeneID: 9255
• UniProt Accession #: Q12904; Q6FG28; Q96CQ9; B3KTR2; B4E1S7
• Molecular Weight: 34,353 Da
• NCBI Official Full Name: endothelial-monocyte activating polypeptide II
• NCBI Official Synonym Full Names: aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
• NCBI Official Symbol: AIMP1
• NCBI Official Synonym Symbols: p43; HLD3; EMAP2; SCYE1; EMAPII
• NCBI Protein Information: aminoacyl tRNA synthase complex-interacting multifunctional protein 1; OTTHUMP00000161877; OTTHUMP00000161878; OTTHUMP00000219673; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; multisynthase complex auxiliary component p43; endothelial-monocyte activating polypeptide II; multisynthetase complex auxiliary component p43; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
• UniProt Protein Name: Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
• Protein Family: Aminoacyl tRNA synthase complex-interacting multifunctional protein
• UniProt Gene Name: AIMP1
• UniProt Synonym Gene Names: EMAP2; SCYE1
• UniProt Entry Name: AIMP1_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq]

Uniprot Description

SCYE1 iso2: Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1- mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7. Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: Golgi apparatus; extracellular space; aminoacyl-tRNA synthetase multienzyme complex; transport vesicle; cell surface; membrane; endoplasmic reticulum; cytoplasm; nucleus; cytosol

Molecular Function: methionine-tRNA ligase activity; protein binding; protein homodimerization activity; cytokine activity; tRNA binding

Biological Process: apoptosis; methionyl-tRNA aminoacylation; glucose metabolic process; signal transduction; chemotaxis; tRNA aminoacylation for protein translation; cell-cell signaling; negative regulation of endothelial cell proliferation; response to wounding; gene expression; angiogenesis; inflammatory response; cell adhesion; leukocyte migration

Disease: Leukodystrophy, Hypomyelinating, 3