Mouse anti-Rat Endothelial cell antigen Monoclonal Antibody

Endothelial cell antigen, Rat, mAb RECA-1

Cat. Num. AAA584213

• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: Endothelial cell antigen; Monoclonal Antibody; Rat; mAb RECA-1; anti-Endothelial cell antigen antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)

• Applicable Applications for anti-Endothelial cell antigen antibody: F, Flow Cytometry (FC/FACS), Immunofluorescence (IF), P
• Application Notes: Application Notes: FC: Standard procedure; Application Use: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Positive Control: Glomerular endothelial cells, RHEC cell line
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-Endothelial cell antigen antibody

Product Description: The monoclonal antibody RECA-1 reacts with Rat Endothelial Cell Antigen (RECA), a cell surface antigen (MCA970R) on rat endothelial cells. Endothelial cells (EC) line the interior of all blood vessels and are the key players in the angiogenesis cascade. EC are the first cells and barrier that vehicles or medicines encounter after systemic delivery. Furthermore, they have a signaling function to the cells of the immune system to indicate the status of the surrounding tissue. RECA-1is at least reactive with the rat MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of the RECA-1 monoclonal antibody was seen with other cell types e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens

References

1. Duijvesteijn, AM et al; Antibodies defining rat endothelial cells: RECA-1, a pan-endothelial cell-specific monoclonal antibody. Lab Invest 1992, 66: 459 2. Iruela-Arispe, L et al; Repair of glomerulonephritis. Am J Pathol 1995, 147: 1715
3. Derhaag, JG et al; Heart EC respond heterogeneous on cytokine stimulation in ICAM-1 and VCAM-1, but not in MHC expression. Endothelium 1997, 5: 307
4. Johnson, RJ et al; Renal injury and salt-sensitive hypertension after exposure to catecholamines. Hypertension, 1999, 34: 151
5. Pradhan, A et al; Heme-oxygenase upregulation ameliorates angiotensin ll-induced tubulointerstitial injury and salt-sensitive hypertension. Am J Nephrol 2006, 26: 552
6. Louboutin, JP et al; Blood-brain barrier abnormalities caused by exposure to HIV-1 gp120-protection by gene delivery of antioxidant enzymes. Neurobiol Dis 2010, 38: 313