Mouse anti-Human Endoglin/CD105 Monoclonal Antibody | anti-ENG antibody

Anti-Endoglin/CD105 Antibody, Mouse Monoclonal

Cat. Num. AAA8101308

• Gene Names: ENG; END; HHT1; ORW1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A
• Synonyms: Endoglin/CD105; Monoclonal Antibody; Anti-Endoglin/CD105 Antibody; Mouse Monoclonal; Endoglin/CD105 Antibody; Mouse MAb; endoglin; Anti-END Antibody; Anti-HHT1 Antibody; Anti-ORW1 Antibody; anti-ENG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 12
• Specificity: Human Endoglin/CD105
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ENG antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:1000-1:2000; IHC-P: 1:200-1:2000
• Immunogen: Recombinant Human Endoglin/CD105 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Endoglin/CD105 (rh Endoglin/CD105). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CD105 in human renal carcinoma with mouse monoclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human CD105 in human tonsil with mouse monoclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human CD105 in human liver with mouse monoclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-ENG antibody

Endoglin, also known as CD15, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,, suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-beta superfamily ligands, endoglin binds TGF-beta1 and TGF-beta3 with high affinity not by itself but by associating with TGF-beta type II receptor (TbetaRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.

Product Categories/Family for anti-ENG antibody

Hemangioblast Markers Cancer Drug Targets

References

Bellon T., et al., (1993), Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur. J. Immunol. 23:2340-2345.Humphray S.J., et al., (2004), DNA sequence and analysis of human chromosome 9.Nature 429:369-374.Gougos A., et al., (1990), Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells.J. Biol. Chem. 265:8361-8364.

NCBI and Uniprot Product Information

• NCBI GI #: 168693647
• NCBI GeneID: 2022
• NCBI Accession #: NP_001108225.1
• NCBI GenBank Nucleotide #: NM_001114753.2
• Molecular Weight: 67,542 Da
• NCBI Official Full Name: endoglin isoform 1
• NCBI Official Synonym Full Names: endoglin
• NCBI Official Symbol: ENG
• NCBI Official Synonym Symbols: END; HHT1; ORW1
• NCBI Protein Information: endoglin; CD105 antigen
• Protein Family: Endoglin

NCBI Description

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]