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Flow Cytometry (Flow Cytometric analysis using ELK3 antibodyFlow Cytometric analysis of HEK293T cells transfected with either recombinant ELK3 protein (red) or empty vector (blue) stained using ELK3 antibody)

Mouse ELK3 Monoclonal Antibody | anti-ELK3 antibody

ELK3 antibody

Gene Names
ELK3; ERP; NET; SAP2; SAP-2
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
ELK3 antibody was purified by affinity chromatography.
Synonyms
ELK3; Monoclonal Antibody; ELK3 antibody; Monoclonal ELK3; Anti-ELK3; SAP2; NET; ERP; Serum response factor accessory protein 2; SAP-2; ETS-related protein ERP; ELK 3; ELK-3; anti-ELK3 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2C10
Purity/Purification
ELK3 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
407
Applicable Applications for anti-ELK3 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:150
WB: 1:2000
Biological Significance
ELK3 is a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor tobind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present.
Cross-Reactivity
Human
Immunogen
ELK3 antibody was raised in mouse using a full length recombinant protein of human ELK3 (NP_005221) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry

(Flow Cytometric analysis using ELK3 antibodyFlow Cytometric analysis of HEK293T cells transfected with either recombinant ELK3 protein (red) or empty vector (blue) stained using ELK3 antibody)

Flow Cytometry (Flow Cytometric analysis using ELK3 antibodyFlow Cytometric analysis of HEK293T cells transfected with either recombinant ELK3 protein (red) or empty vector (blue) stained using ELK3 antibody)

Immunofluorescence

(Immunofluorescent staining using ELK3 antibodyImmunofluorescent staining of COS7 cells transiently transfected with recombinant ELK3 protein using ELK3 antibody)

Immunofluorescence (Immunofluorescent staining using ELK3 antibodyImmunofluorescent staining of COS7 cells transiently transfected with recombinant ELK3 protein using ELK3 antibody)

Immunohistochemistry

(Immunohistochemical staining using ELK3 antibodyImmunohistochemical analysis of ELK3 protein in paraffin embedded Carcinoma of Human bladder tissue using ELK3 antibody)

Immunohistochemistry (Immunohistochemical staining using ELK3 antibodyImmunohistochemical analysis of ELK3 protein in paraffin embedded Carcinoma of Human bladder tissue using ELK3 antibody)

Western Blot

(Western Blot analysis using ELK3 antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ELK3 protein (Right) or empty vector (Left) detected with ELK3 antibody)

Western Blot (Western Blot analysis using ELK3 antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ELK3 protein (Right) or empty vector (Left) detected with ELK3 antibody)
Related Product Information for anti-ELK3 antibody
Mouse monoclonal ELK3 antibody
Product Categories/Family for anti-ELK3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,240 Da
NCBI Official Full Name
ETS domain-containing protein Elk-3 isoform 1
NCBI Official Synonym Full Names
ELK3, ETS-domain protein (SRF accessory protein 2)
NCBI Official Symbol
ELK3
NCBI Official Synonym Symbols
ERP; NET; SAP2; SAP-2
NCBI Protein Information
ETS domain-containing protein Elk-3
UniProt Protein Name
ETS domain-containing protein Elk-3
UniProt Gene Name
ELK3
UniProt Synonym Gene Names
NET; SAP2; SAP-2; SRF accessory protein 2
UniProt Entry Name
ELK3_HUMAN

NCBI Description

This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

ELK3: a transcription factor of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. May be a negative regulator of transcription but can activate transcription when co expressed with Ras, Src or Mos. Activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q23

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; mitochondrion; nucleus

Molecular Function: protein binding; transcription corepressor activity; transcription factor activity; purine-rich negative regulatory element binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; wound healing; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription, DNA-dependent; cell differentiation; signal transduction

Research Articles on ELK3

Similar Products

Product Notes

The ELK3 elk3 (Catalog #AAA830566) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ELK3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the ELK3 elk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELK3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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