Mouse anti-Human Elk-1 Monoclonal Antibody | anti-ELK1 antibody

Elk-1, phosphorylated (Ser383)

Cat. Num. AAA604231

• Reactivity: Human
• Applications: ELISA
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Elk-1; Monoclonal Antibody; phosphorylated (Ser383); Anti -Elk-1; anti-ELK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 9A129
• Specificity: Reactive to the immunogen human Elk-1, phosphorylated [pS383], and peptide-BSA conjugating.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a solution in 0.01M PBS, pH 7.2. Reconstitution: Double distillated water is recommended to adjust the final concentration to 1mg/ml.

• Applicable Applications for anti-ELK1 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA., , Recommended Dilution:, Indirect ELISA: 1ug/ml of peptide/or peptide conjugate in pH 9.5 carbonate buffer, 100ul/well., Optimal dilutions to be determined by the researcher.
• Immunogen: Peptide of a phosphorylated pS383 fragment of ELK-1, conjugated with KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50?, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ELK1 antibody

Elk-1, a 62kD transcription factor, is the most abundant component of ternary complex factors that is regulated by phosphorylation and in turn regulates gene expression in response to multiple stimuli. Elk-1 is a substrate of MAP kinase and phosphorylated by ERK2 on several sites in the carboxyl terminus, including serine 383. Phosphorylation of Elk-1 on serine 383 is critical for its transcriptional activity, including Elk-1-mediated transcription of c-Fos. Dephosphorylation of serine 383 by calcineurin downregulates Elk-1 transcriptional activity.

Product Categories/Family for anti-ELK1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 166362732
• NCBI GeneID: 2002
• NCBI Accession #: NP_001107595.1
• NCBI GenBank Nucleotide #: NM_001114123.1
• UniProt Accession #: P19419; O75606; O95058; Q969X8; Q9UJM4; B2R7H4; Q59GR2; Q6FG56; Q86SR6; Q8N9S0
• Molecular Weight: 44,888 Da
• NCBI Official Full Name: ETS domain-containing protein Elk-1
• NCBI Official Synonym Full Names: ELK1, member of ETS oncogene family
• NCBI Official Symbol: ELK1
• NCBI Protein Information: ETS domain-containing protein Elk-1; ETS-like gene 1; OTTHUMP00000023224; OTTHUMP00000023225
• UniProt Protein Name: ETS domain-containing protein Elk-1
• Protein Family: ETS domain-containing protein
• UniProt Gene Name: ELK1
• UniProt Entry Name: ELK1_HUMAN

NCBI Description

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Uniprot Description

ELK1: a transcription factor of the Ets family and the ternary complex factor (TCF) subfamily. Forms a ternary complex by binding to the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. Mediates gene activity in response to serum and growth factors. Phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites is critical for transcriptional activation by Elk-1. Two alternatively-spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: nucleoplasm; cell soma; mitochondrion; dendrite; nerve terminal; nucleus

Molecular Function: protein binding; double-stranded DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; response to light stimulus; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway