Western Blot (WB)
(EIF4B Antibody western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the EIF4B antibody detected the EIF4B protein (arrow).)
Mouse anti-Human EIF4B Monoclonal Antibody | anti-EIF4B antibody
EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (APC)
Gene Names
EIF4B; EIF-4B; PRO1843
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
EIF4B; Monoclonal Antibody; EIF4B (EIF4B; Eukaryotic translation initiation factor 4B) (APC); Eukaryotic translation initiation factor 4B; anti-EIF4B antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
355CT12.6.5
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-EIF4B antibody
FLISA, Western Blot (WB)
Application Notes
FLISA: 1:1000
WB: 1:100-250
Applications are based on unconjugated antibody.
WB: 1:100-250
Applications are based on unconjugated antibody.
Immunogen
EIF4B Mab is generated from mouse immunized with EIF4B recombinant protein.
Conjugate
APC
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C with glycerol. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze APC conjugates. Light sensitive.
Western Blot (WB)
(EIF4B Antibody western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the EIF4B antibody detected the EIF4B protein (arrow).)
Western Blot (WB)
(EIF4B Antibody western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the EIF4B antibody detected the EIF4B protein (arrow).)
Related Product Information for anti-EIF4B antibody
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Product Categories/Family for anti-EIF4B antibody
References
Mokas, S., et al. Mol. Biol. Cell 20(11):2673-2683(2009) Rozovsky, N., et al. RNA 14(10):2136-2148(2008) Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007) Olsen, J.V., et al. Cell 127(3):635-648(2006) Olsen, J.V., et al. Cell 127(3):635-648(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,151 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4B
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4B
NCBI Official Symbol
EIF4B
NCBI Official Synonym Symbols
EIF-4B; PRO1843
NCBI Protein Information
eukaryotic translation initiation factor 4B
UniProt Protein Name
Eukaryotic translation initiation factor 4B
Protein Family
UniProt Gene Name
EIF4B
UniProt Synonym Gene Names
eIF-4B
UniProt Entry Name
IF4B_HUMAN
Uniprot Description
EIF4B: eukaryotic translation initiation factor 4B. Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Protein type: RNA-binding; Translation initiation; Translation
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: eukaryotic translation initiation factor 4F complex; cytosol
Molecular Function: protein binding; RNA binding; translation initiation factor activity; nucleotide binding
Biological Process: poly(A) tail shortening; cellular protein metabolic process; translation; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; regulation of translational initiation
Research Articles on EIF4B
Similar Products
Product Notes
The EIF4B eif4b (Catalog #AAA6120993) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EIF4B (EIF4B, Eukaryotic translation initiation factor 4B) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF4B can be used in a range of immunoassay formats including, but not limited to, FLISA, Western Blot (WB). FLISA: 1:1000 WB: 1:100-250 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EIF4B eif4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF4B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
