Western Blot (WB)
(Western Blot detection against Immunogen (37.11kD).)
Mouse anti-Human EIF2A Monoclonal Antibody | anti-EIF2A antibody
EIF2A (Eukaryotic Translation Initiation Factor 2A, eIF-2A, 65kD Eukaryotic Translation Initiation Factor 2A, CDA02, MSTP004, MSTP089) APC
Gene Names
EIF2A; CDA02; EIF-2A; MST089; MSTP004; MSTP089
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
EIF2A; Monoclonal Antibody; EIF2A (Eukaryotic Translation Initiation Factor 2A; eIF-2A; 65kD Eukaryotic Translation Initiation Factor 2A; CDA02; MSTP004; MSTP089) APC; anti-EIF2A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3D5
Specificity
Recognizes human EIF2A.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-EIF2A antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa1-101 from human EIF2A (NP_114414) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAPSTPLLTVRGSEGLYMVNGPPHFTESTVFPRESGKNCKVCIFSKDGTLFAWGNGEKVNIISVTNKGLLHSFDLLKAVCLEFSPKNTVLATWQPYTTSK
Conjugate
APC
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (37.11kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37.11kD).)
Western Blot (WB)
(Western Blot analysis of EIF2A expression in transfected 293T cell line by EIF2A monoclonal antibody. Lane 1: EIF2A transfected lysate (65kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of EIF2A expression in transfected 293T cell line by EIF2A monoclonal antibody. Lane 1: EIF2A transfected lysate (65kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-EIF2A antibody
EIF-2alpha, also known as eukaryotic translation initiation factor 2alpha, promotes the binding of the initiator tRNA to the 40S ribosomal subunits and controls translational rates via phosphorylation/dephosphorylation mechanisms. The double-stranded RNA (dsRNA)-activated protein kinase (PKR) provides an important control in the regulation of protein synthesis initiation through the phosphorylation of eIF-2alpha. Phosphorylated eIF-2 alpha inhibits cell growth and induces apoptosis. Phosphorylation of eIF-2alpha occurs under a variety of conditions including viral infection, apoptosis, nutrient deprivation, heme-deprivation, and certain stresses. EIF-2 exists as a heterotrimeric protein composed of 3 nonidentical subunits, alpha (36kD), beta (38kD), and gamma (52kD).
Product Categories/Family for anti-EIF2A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,994 Da
NCBI Official Full Name
eukaryotic translation initiation factor 2A
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 2A, 65kDa
NCBI Official Symbol
EIF2A
NCBI Official Synonym Symbols
CDA02; EIF-2A; MST089; MSTP004; MSTP089
NCBI Protein Information
eukaryotic translation initiation factor 2A; 65 kDa eukaryotic translation initiation factor 2A
UniProt Protein Name
Eukaryotic translation initiation factor 2A
Protein Family
UniProt Gene Name
EIF2A
UniProt Synonym Gene Names
eIF-2A
UniProt Entry Name
EIF2A_HUMAN
Uniprot Description
EIF2A: Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. Belongs to the WD repeat EIF2A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Translation
Chromosomal Location of Human Ortholog: 3q25.1
Cellular Component: extracellular space; cytoplasm; eukaryotic translation initiation factor 2 complex
Molecular Function: protein binding; translation initiation factor activity; ribosome binding; tRNA binding
Biological Process: regulation of translation; ribosome assembly; positive regulation of signal transduction; translational initiation; SREBP-mediated signaling pathway; protein amino acid phosphorylation
Similar Products
Product Notes
The EIF2A eif2a (Catalog #AAA6136380) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The EIF2A (Eukaryotic Translation Initiation Factor 2A, eIF-2A, 65kD Eukaryotic Translation Initiation Factor 2A, CDA02, MSTP004, MSTP089) APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EIF2A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EIF2A eif2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF2A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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