Immunofluorescence (IF)
(Immunofluorescence showing EEF1G in HeLa cells using MBS605232 (10ug/ml).)
Mouse anti-Human eEF1G Monoclonal Antibody | anti-EEF1G antibody
eEF1G (Eukaryotic Translation Elongation Factor 1 gamma, Elongation Factor 1g, eEF-1B gamma, EF1 gamma, EF-1-gamma, EF1G, 2610301D06Rik, GIG35, MGC94929, MGC103354, MGC114210, MGC144723, MGC144724, Translation Elongation Factor eEF-1 gamma Chain) (Azide F
Gene Names
EEF1G; EF1G; GIG35
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
eEF1G; Monoclonal Antibody; eEF1G (Eukaryotic Translation Elongation Factor 1 gamma; Elongation Factor 1g; eEF-1B gamma; EF1 gamma; EF-1-gamma; EF1G; 2610301D06Rik; GIG35; MGC94929; MGC103354; MGC114210; MGC144723; MGC144724; Translation Elongation Factor eEF-1 gamma Chain) (Azide F; Anti -eEF1G (Eukaryotic Translation Elongation Factor 1 gamma; anti-EEF1G antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10C41
Specificity
Recognizes human EEF1G. Species Crossreactivity: mouse, rat.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free).
Applicable Applications for anti-EEF1G antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Immunofluorescence and Western Blot.
Dilution: Western Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Dilution: Western Blot: 1:500-1:1000
ELISA: 1ug/ml-3ng/ml
Immunogen
Recombinant protein corresponding to aa1-438 of human EEF1G.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunofluorescence (IF)
(Immunofluorescence showing EEF1G in HeLa cells using MBS605232 (10ug/ml).)
Immunofluorescence (IF)
(Immunofluorescence showing EEF1G in HeLa cells using MBS605232 (10ug/ml).)
Western Blot (WB)
(Western Blot analysis of EEF1G over-expressed 293 cell line, cotransfected with EEF1G Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MBS605232. GAPDH ( 36.1kD) used as specificity and loading control.)
Western Blot (WB)
(Western Blot analysis of EEF1G over-expressed 293 cell line, cotransfected with EEF1G Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MBS605232. GAPDH ( 36.1kD) used as specificity and loading control.)
Related Product Information for anti-EEF1G antibody
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.
Product Categories/Family for anti-EEF1G antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50,119 Da
NCBI Official Full Name
EEF1G
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 gamma
NCBI Official Symbol
EEF1G
NCBI Official Synonym Symbols
EF1G; GIG35
NCBI Protein Information
elongation factor 1-gamma; PRO1608; EF-1-gamma; eEF-1B gamma; OTTHUMP00000236167; pancreatic tumor-related protein; translation elongation factor eEF-1 gamma chain
UniProt Protein Name
EEF1G protein
Protein Family
UniProt Gene Name
EEF1G
UniProt Synonym Gene Names
EF1G
UniProt Entry Name
Q53YD7_HUMAN
NCBI Description
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases. [provided by RefSeq]
Uniprot Description
EEF1G: Probably plays a role in anchoring the complex to other cellular components.
Protein type: Translation; Translation elongation
Chromosomal Location of Human Ortholog: 11q12.3
Cellular Component: membrane; endoplasmic reticulum; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; translation elongation factor activity
Biological Process: translational elongation; cellular protein metabolic process; translation; response to virus; gene expression
Research Articles on EEF1G
Similar Products
Product Notes
The EEF1G eef1g (Catalog #AAA605232) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The eEF1G (Eukaryotic Translation Elongation Factor 1 gamma, Elongation Factor 1g, eEF-1B gamma, EF1 gamma, EF-1-gamma, EF1G, 2610301D06Rik, GIG35, MGC94929, MGC103354, MGC114210, MGC144723, MGC144724, Translation Elongation Factor eEF-1 gamma Chain) (Azide F reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's eEF1G can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in ELISA, Immunofluorescence and Western Blot. Dilution: Western Blot: 1:500-1:1000 ELISA: 1ug/ml-3ng/ml. Researchers should empirically determine the suitability of the EEF1G eef1g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eEF1G, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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