Western Blot (WB)
(Western blot detection of ENC-1 expression in human neuroblastoma cell lysate using MBS605906.)
Mouse anti-Human Ectodermal-neural Cortex Protein 1 Monoclonal Antibody | anti-ENC1 antibody
Ectodermal-neural Cortex Protein 1 (ENC-1, p53-induced Gene 10 Protein, Nuclear Matrix Protein NRP/B, NRPB, PIG10)
Purified by affinity chromatography.
Purified by affinity chromatography.
Western Blot (WB)
(Western blot detection of ENC-1 expression in human neuroblastoma cell lysate using MBS605906.)
Western Blot (WB)
(Western blot detection of ENC-1 expression in human neuroblastoma cell lysate using MBS605906.)
ELISA (EIA)
(Sandwich ELISA using MBS605906 as a capture antibody. The detection limit for GST tagged recombinant ENC-1 is 0.3 ng/ml.)
ELISA (EIA)
(Sandwich ELISA using MBS605906 as a capture antibody. The detection limit for GST tagged recombinant ENC-1 is 0.3 ng/ml.)
NCBI and Uniprot Product Information
NCBI Description
DNA damage and/or hyperproliferative signals activate wildtype p53 tumor suppressor protein (TP53; MIM 191170), inducing cell cycle arrest or apoptosis. Mutations that inactivate p53 occur in 50% of all tumors. Polyak et al. (1997) [PubMed 9305847] used serial analysis of gene expression (SAGE) to evaluate cellular mRNA levels in a colorectal cancer cell line transfected with p53. Of 7,202 transcripts identified, only 14 were expressed at levels more than 10-fold higher in p53-expressing cells than in control cells. Polyak et al. (1997) [PubMed 9305847] termed these genes 'p53-induced genes,' or PIGs, several of which were predicted to encode redox-controlling proteins. They noted that reactive oxygen species (ROS) are potent inducers of apoptosis. Flow cytometric analysis showed that p53 expression induces ROS production, which increases as apoptosis progresses under some conditions. The authors stated that the PIG10 gene, also called ENC1, encodes an actin-binding protein.[supplied by OMIM]
Uniprot Description
ENC1: Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. May be down-regulated in neuroblastoma tumors. Substrate- specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Protein type: Actin-binding
Chromosomal Location of Human Ortholog: 5q13
Cellular Component: nucleoplasm; cytoskeleton; nuclear matrix; cell soma; nuclear chromatin; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; actin binding
Biological Process: nervous system development; negative regulation of translation; multicellular organismal development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination
Research Articles on ENC1
2. NRP/B mutations impair Nrf2-dependent NQO1 induction in primary brain tumors.
3. NRP/B enhances oxidative stress responses in breast cancer cells via the Nrf2 pathway, identifying a novel role of nuclear matrix protein(s) in oxidative stress responses.
4. results suggest that upregulation of ENC-1 contributes to the development of HCL and provides new information on the possible dysregulation of ENC-1 including expression of a novel antisense gene, ENC-1AS
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Product Notes
The ENC1 enc1 (Catalog #AAA605906) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Ectodermal-neural Cortex Protein 1 (ENC-1, p53-induced Gene 10 Protein, Nuclear Matrix Protein NRP/B, NRPB, PIG10) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ectodermal-neural Cortex Protein 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the ENC1 enc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ectodermal-neural Cortex Protein 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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