Western Blot (WB)
(Western Blot analysis of EBF1 expression in transfected 293T cell line by EBF1 monoclonal antibody (M01), clone 1C12.Lane 1: EBF1 transfected lysate (64 KDa).Lane 2: Non-transfected lysate.)
Mouse EBF1 Monoclonal Antibody | anti-EBF1 antibody
EBF1 (Early B-Cell Factor 1, COE1, EBF, FLJ39389, FLJ41763, O/E-1, OLF1) (FITC)
Gene Names
EBF1; EBF; COE1; OLF1; O/E-1
Applications
Western Blot
Purity
Purified
Synonyms
EBF1; Monoclonal Antibody; EBF1 (Early B-Cell Factor 1; COE1; EBF; FLJ39389; FLJ41763; O/E-1; OLF1) (FITC); Early B-Cell Factor 1; OLF1; anti-EBF1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1C12
Specificity
Recognizes EBF1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Sequence Length
591
Applicable Applications for anti-EBF1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
EBF1 (AAH38805, 1aa-591aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFSKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQLPALANTSVHAGMMGVNSFSGQLAVNVSEASQATNQGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNGYGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAPVVRPQTSPPPTCTSTNGNSLQAISGMIVPPM
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of EBF1 expression in transfected 293T cell line by EBF1 monoclonal antibody (M01), clone 1C12.Lane 1: EBF1 transfected lysate (64 KDa).Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of EBF1 expression in transfected 293T cell line by EBF1 monoclonal antibody (M01), clone 1C12.Lane 1: EBF1 transfected lysate (64 KDa).Lane 2: Non-transfected lysate.)
Related Product Information for anti-EBF1 antibody
Mouse monoclonal antibody raised against a full length recombinant EBF1.
Product Categories/Family for anti-EBF1 antibody
References
1. Early B-Cell Factor-1 (Ebf1) is a key regulator of metabolic and inflammatory signaling pathways in mature adipocytes.Griffin MJ, Zhou Y, Kang S, Zhang X, Mikkelsen TS, Rosen EDJ Biol Chem. 2013 Oct 30. 2.Cellular origin and pathophysiology of chronic lymphocytic leukemia. Seifert M, Sellmann L, Bloehdorn J, Wein F, Stilgenbauer S, Durig J, Kuppers R.J Exp Med. 2012 Oct 22.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Early B-cell factor 1
NCBI Official Synonym Full Names
EBF transcription factor 1
NCBI Official Symbol
EBF1
NCBI Official Synonym Symbols
EBF; COE1; OLF1; O/E-1
NCBI Protein Information
transcription factor COE1
Protein Family
Research Articles on EBF1
Similar Products
Product Notes
The EBF1 (Catalog #AAA6176221) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's EBF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EBF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EBF1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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