Typical Testing Data/Standard Curve (for reference only)
Monkey Ribonuclease Inhibitor ELISA Kit | RI elisa kit
Monkey Ribonuclease Inhibitor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This RI ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey RI. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY RI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RI antibody and an RI-HRP conjugate. The assay sample and buffer are incubated together with RI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RI concentration since RI from samples and RI-HRP conjugate compete for the anti-RI antibody binding site. Since the number of sites is limited, as more sites are occupied by RI from the sample, fewer sites are left to bind RI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RI concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]
Uniprot Description
RNH1: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.
Protein type: Inhibitor
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: angiogenin-PRI complex; cytoplasm
Molecular Function: protein binding; ribonuclease inhibitor activity
Biological Process: negative regulation of catalytic activity; mRNA catabolic process; regulation of angiogenesis
Research Articles on RI
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Product Notes
The Monkey RI rnh1 (Catalog #AAA741409) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741409 ELISA Kit recognizes Monkey RI. It is sometimes possible for the material contained within the vial of "Ribonuclease Inhibitor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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