Rat EB1 Monoclonal Antibody | anti-EB1 antibody

Anti-EB1 Antibody

Cat. Num. AAA417526

• Gene Names: MAPRE1; EB1
• Reactivity: Mouse, Human, Ape, Hamster
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: EB1; Monoclonal Antibody; Anti-EB1 Antibody; Anti-mouse human ape and hamster EB1 Antibody; anti-EB1 antibody

• Host: Rat
• Reactivity: Mouse, Human, Ape, Hamster
• Clonality: Monoclonal
• Isotype: IgG2a
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 268

• Applicable Applications for anti-EB1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), Immunofluorescent staining
• Application Notes: WB 1:2000; ELISA 1:2000; Immunoprecipitation 1:2000; Immunofluorescent staining 1:200
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Immunogen: The C-terminal part of mouse EB1 (amino acids 125-268) fused to GST
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

References

Komarova Y, Lansbergen G, Galjart N, Grosveld F, Borisy GG and Akhmanova A. 2005. EB1 and EB3 Control CLIP Dissociation from the Ends of Growing Microtubules. Mol. Biol. Cell. 16:5334-45.

NCBI and Uniprot Product Information

• NCBI GI #: 998357
• NCBI GeneID: 22919
• Molecular Weight: 29,999 Da
• NCBI Official Full Name: EB1
• NCBI Official Synonym Full Names: microtubule-associated protein, RP/EB family, member 1
• NCBI Official Symbol: MAPRE1
• NCBI Official Synonym Symbols: EB1
• NCBI Protein Information: microtubule-associated protein RP/EB family member 1; APC-binding protein EB1; adenomatous polyposis coli-binding protein EB1; end-binding protein 1
• UniProt Protein Name: Microtubule-associated protein RP/EB family member 1
• UniProt Gene Name: MAPRE1
• UniProt Entry Name: MARE1_HUMAN

NCBI Description

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]

Uniprot Description

EB1: a microtubule-associated protein of the RP/EB family. Binds to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. Localizes to microtubules, especially the growing ends, in interphase cells. Associates with the centrosomes and spindle microtubules during mitosis. Also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. May be involved in the regulation of microtubule structures and chromosome stability.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 20q11.1-q11.23

Cellular Component: Golgi apparatus; microtubule; centrosome; cytoplasmic microtubule; cell projection membrane; cytoplasm; cortical microtubule cytoskeleton; spindle; cytosol

Molecular Function: protein C-terminus binding; microtubule plus-end binding; protein binding

Biological Process: cell proliferation; mitosis; cell division; organelle organization and biogenesis; negative regulation of microtubule polymerization; mitotic cell cycle; G2/M transition of mitotic cell cycle